To study the immune effect of recombinant avian influenza virus H5 subtype bivalent inactivated vaccine( H5N1,Re-6 strain + Re-4 strain) and to provide the basis for formulating reasonable immune procedure of avian influenza vaccine in clinical practice. A total of 12 batches of vaccines from three companies were used for the immune of SPF chickens and SPF ducks. Each chicken or duck serum was separately collected every 3 weeks until the immunization up to the 24 th week. The serum antibody titers of Re-6 and Re-4 were detected. The results showed that the HI titers of the inoculated SPF chickens and SPF ducks reached the peak when the immune time were the 6thand 3rdweek after the first immunization respectively; then the titer decreased gradually as time prolonged; the highest titer of SPF chickens was greater than that of SPF ducks; the high titer duration of SPF chickens were longer than that of SPF ducks; and all the vaccines from the three companies showed a good immune effect.
展开▼
Institute of Poultry Science, Shandong Academy of Agricultural Sciences/Key Laboratory of Diagnosis and Immunity of Poultry Diseases in Shandong Province, Ji-nan 250023, China;
