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首页> 中文期刊> 《动物与饲料科学：英文版》 >Biological Characterization and Whole Genome Sequencing of a Genotype VII Newcastle Disease Virus Strain

Biological Characterization and Whole Genome Sequencing of a Genotype VII Newcastle Disease Virus Strain

         
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[Objective]The aim of this paper was to study the genotype,pathogenicity and nucleotide difference between Newcastle disease virus(NDV)isolates and traditional NDV vaccine strain(La Sota).[Method]A suspected NDV strain was isolated from a chicken farm.The isolate was preliminarily determined by HA and HI tests.A pair of primers was designed based on the partial sequence of NDV F gene published in GenBank(accession No.JF950510.1).F gene was amplified by RT-PCR,cloned and sequenced.The sequencing result was compared with the F gene sequences published in GenBank,and the phylogenetic tree was constructed to analyze the genotypes.The pathogenicity of the virus was determined by mean death time(MDT)of chicken embryos,intracerebral pathogenicity index(ICPI)of one-day-old chicks and intravenous pathogenicity index(IVPI)of six-week-old chickens,respectively.Based on the NDV genome sequence published in GenBank(accession No.JF950510.1),nine pairs of primers were designed to amplify the genome sequence of the isolate,and its structure was analyzed.[Result]The length of F gene was about 500 bp,and a NDV strain of genotype VII was isolated.The MDT,ICPI and IVPI were 52.8 h,1.675 and 2.46,respectively,indicating the isolate was a virulent strain.The whole genome sequence analysis results showed that the full genome length of the isolate was 15 192 bp,which had 6 more nu-cleotides than that of La Sota strain,and the homology between the two strains was 82.8%.[Conclusion]A virulent NDV strain of genotype VII was isolated,with low homology to La Sota strain in nucleotide sequence.

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