Arginine, N-carbamylglutamate,and glutamine exert protective effects against oxidative stress in rat intestine

Liang Xiao; Wei Cao; Guangmang Liu; Tingting Fang; Xianjian Wu; Gang Jia; Xiaoling Chen; Hua Zhao; Jing Wang; Caimei Wu; Jingyi Cai

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Arginine, N-carbamylglutamate,and glutamine exert protective effects against oxidative stress in rat intestine

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The objective of the current study is to evaluate the effects of dietary supplementation with arginine(ARG), N-carbamylglutamate(NCG), and glutamine(GLN) on rat intestinal morphology and antioxidant status under oxidative stress. Rats were fed for 30 d with one of the following iso-nitrogenous diets:basal diet(BD), BD plus 1% ARG, BD plus 0.1% NCG, and BD plus 1% GLN. On day 28, half of the rats fed BD were intraperitoneally injected with 12 mg/kg body weight of diquat(DT; i.e., the DT group) and the other half was intraperitoneally injected with sterile solution(i,e., the control group). The other diet groups were intraperitoneally injected with 12 mg/kg body weight of DT(i,e., DT + 1% GLN [DT + GLN],DT + 1% ARG [DT + ARG], and DT + 0.1% NCG [DT + NCG]). Rat jejunum samples obtained at 48 h after DT injection were analyzed. Results showed that DT significantly decreased catalase(CAT) activity and glutathione(GSH) content by 58.25% and 56.57%, respectively, and elevated malondialdehyde(MDA)content and crypt depth(CD) by 19.39% and 22.13%, respectively, in the jejunum(P < 0.05, relative to the control group). Compared with the DT group, the DT + GLN group exhibited significantly improved villus height(VH), villus width(VW), villus surface area(VSA), CD and total antioxidant capacity(T-AOC) activity(P < 0.05); the DT + ARG group exhibited significantly increased the ratio of VH to CD(H:D) and TAOC activity(P < 0.05); the DT + GLN, DT + ARG and DT + NCG groups exhibited significantly enhanced CAT activity and GSH content as well as decreased MDA content(P < 0.05). Moreover, VH, VW, VSA, CD and GSH content in the DT + GLN group were higher whereas MDA content was lower compared with the corresponding values observed in both the DT + ARG and the DT + NCG groups(P < 0.05). The H:D ratio in the DT + ARG group significantly increased compared with that in the DT + NCG and DT + GLN groups(P < 0.05). Collectively, this study suggested that dietary supplementation with 1% GLN, 0.1% NCG,and 1% ARG was effective in enhancing the antioxidant status and maintaining the morphological structure of rat jejunum under oxidative stress; of these supplements, 1% GLN exerted the greatest effects on mitigating oxidative stress.

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《动物营养：英文版》 |2016年第3期|P.242-248|共7页
作者
Liang Xiao; Wei Cao; Guangmang Liu; Tingting Fang; Xianjian Wu; Gang Jia; Xiaoling Chen; Hua Zhao; Jing Wang; Caimei Wu; Jingyi Cai;
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作者单位

Institute of Animal Nutrition, Sichuan Agicultural University;

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education;

Maize Research Institute, Sichuan Agricultural University;

Institute of Animal Nutrition, Sichuan Agicultural University;

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education;

Maize Research Institute, Sichuan Agricultural University;

Institute of Animal Nutrition, Sichuan Agicultural University;

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education;

Maize Research Institute, Sichuan Agricultural University;

Institute of Animal Nutrition, Sichuan Agicultural University;

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education;

Maize Research Institute, Sichuan Agricultural University;

Institute of Animal Nutrition, Sichuan Agicultural University;

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education;

Maize Research Institute, Sichuan Agricultural University;

Institute of Animal Nutrition, Sichuan Agicultural University;

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education;

Maize Research Institute, Sichuan Agricultural University;

Institute of Animal Nutrition, Sichuan Agicultural University;

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education;

Maize Research Institute, Sichuan Agricultural University;

Institute of Animal Nutrition, Sichuan Agicultural University;

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education;

Maize Research Institute, Sichuan Agricultural University;

Institute of Animal Nutrition, Sichuan Agicultural University;

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education;

Maize Research Institute, Sichuan Agricultural University;

Institute of Animal Nutrition, Sichuan Agicultural University;

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education;

Maize Research Institute, Sichuan Agricultural University;

Institute of Animal Nutrition, Sichuan Agicultural University;

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education;

Maize Research Institute, Sichuan Agricultural University;

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正文语种 CHI
中图分类家畜生理学、生物物理学、生物化学;
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Arginine, N-carbamylglutamate,and glutamine exert protective effects against oxidative stress in rat intestine

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