Gut microbiota absence and transplantation affect growth and intestinal functions:An investigation in a germ-free pig model

Hua Zhou; Jing Sun; Bing Yu; Zuohua Liu; Hong Chen; Jun He; Xiangbing Mao; Ping Zheng; Jie Yu; Junqiu Luo; Yuheng Luo; Hui Yan; Liangpeng Ge; Daiwen Chen

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Gut microbiota absence and transplantation affect growth and intestinal functions:An investigation in a germ-free pig model

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This study was conducted to investigate host-microbiota interactions and explore the effects of maternal gut microbiota transplantation on the growth and intestinal functions of newborns in a germ-free(GF)pig model.Twelve hysterectomy-derived GF Bama piglets were reared in 6 sterile isolators.Among them,6 were considered as the GF group,and the other 6 were orally inoculated with healthy sow fecal suspension as fecal microbiota transplanted(FMT)group.Another 6 piglets from natural birth were regarded as the conventional(CV)group.The GF and FMT groups were hand-fed with Co60-y-irradiated sterile milk powder,while the CV group was reared by lactating Bama sows.All groups were fed for 21 days.Then,all piglets and then were switched to sterile feed for another 21 days.Results showed that the growth performance,nutrient digestibility,and concentrations of short-chain fatty acids in the GF group decreased(P<0.05).Meanwhile,the serum urea nitrogen concentration and digesta pH values in the GF group increased compared with those in the FMT and CV groups(P<0.05).Compared with the CV group,the GF group demonstrated upregulation in the mRNA expression levels of intestinal barrier function-related genes in the small intestine(P<0.05).In addition,the mRNA abundances of intestinal development and absorption-related genes in the small intestine and colon were higher in the GF group than in the CV and FMT groups(P<0.05).The FMT group exhibited greater growth performance,lipase activity,and nutrient digestibility(P<0.05),higher mRNA expression levels of intestinal development and barrier-related genes in the small intestine(P<0.05),and lower mRNA abundances of pro-inflammatory factor in the colon and jejunum(P<0.05)than the CV group.In conclusion,the absence of gut microbes impaired the growth and nutrient digestibility,and healthy sow gut microbiota transplantation increased the growth and nutrient digestibility and improved the intestinal development and barrier function of newborn piglets,indicating the importance of intestinal microbes for intestinal development and functions.

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《动物营养：英文版》 |2021年第2期|P.295-304|共10页
作者
Hua Zhou; Jing Sun; Bing Yu; Zuohua Liu; Hong Chen; Jun He; Xiangbing Mao; Ping Zheng; Jie Yu; Junqiu Luo; Yuheng Luo; Hui Yan; Liangpeng Ge; Daiwen Chen;
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Key Laboratory of Animal Disease-Resistance Nutrition Chengdu Sichuan 611130 ChinaAnimal Nutrition Institute Sichuan Agricultural University Chengdu Sichuan 611130 China;

Key Laboratory of Pig Industry Sciences Rongchang Chongqing 402460 ChinaChongqing Academy of Animal Sciences Rongchang Chongqing 402460 China;

Key Laboratory of Animal Disease-Resistance Nutrition Chengdu Sichuan 611130 ChinaAnimal Nutrition Institute Sichuan Agricultural University Chengdu Sichuan 611130 China;

Key Laboratory of Pig Industry Sciences Rongchang Chongqing 402460 ChinaChongqing Academy of Animal Sciences Rongchang Chongqing 402460 China;

College of Food Science Sichuan Agricultural University Ya''an Sichuan 625014 China;

Key Laboratory of Animal Disease-Resistance Nutrition Chengdu Sichuan 611130 ChinaAnimal Nutrition Institute Sichuan Agricultural University Chengdu Sichuan 611130 China;

Key Laboratory of Animal Disease-Resistance Nutrition Chengdu Sichuan 611130 ChinaAnimal Nutrition Institute Sichuan Agricultural University Chengdu Sichuan 611130 China;

Key Laboratory of Animal Disease-Resistance Nutrition Chengdu Sichuan 611130 ChinaAnimal Nutrition Institute Sichuan Agricultural University Chengdu Sichuan 611130 China;

Key Laboratory of Animal Disease-Resistance Nutrition Chengdu Sichuan 611130 ChinaAnimal Nutrition Institute Sichuan Agricultural University Chengdu Sichuan 611130 China;

Key Laboratory of Animal Disease-Resistance Nutrition Chengdu Sichuan 611130 ChinaAnimal Nutrition Institute Sichuan Agricultural University Chengdu Sichuan 611130 China;

Key Laboratory of Animal Disease-Resistance Nutrition Chengdu Sichuan 611130 ChinaAnimal Nutrition Institute Sichuan Agricultural University Chengdu Sichuan 611130 China;

Key Laboratory of Animal Disease-Resistance Nutrition Chengdu Sichuan 611130 ChinaAnimal Nutrition Institute Sichuan Agricultural University Chengdu Sichuan 611130 China;

Key Laboratory of Pig Industry Sciences Rongchang Chongqing 402460 ChinaChongqing Academy of Animal Sciences Rongchang Chongqing 402460 China;

Key Laboratory of Animal Disease-Resistance Nutrition Chengdu Sichuan 611130 ChinaAnimal Nutrition Institute Sichuan Agricultural University Chengdu Sichuan 611130 China;

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原文格式 PDF
正文语种 chi
中图分类消化系及腹部疾病;
关键词
Germ-free; Host microbiota interaction; Maternal gut microbiota transplantation; Growth performance; Intestinal function; Pig model;

Gut microbiota absence and transplantation affect growth and intestinal functions:An investigation in a germ-free pig model

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