Effect of L-carnitine supplementation during in vitro maturation and in vitro culture on oocyte quality and embryonic development rate of bovines

Diego F. Carrillo-González; Nélida Rodríguez-Osorio; Charles R. Long; Neil A. Vásquez-Araque; Juan G. Maldonado-Estrada

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Effect of L-carnitine supplementation during in vitro maturation and in vitro culture on oocyte quality and embryonic development rate of bovines

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Objective:To assess the effect of L-carnitine supplementation during in vitro oocyte maturation and in vitro culture process of bovine oocytes.Methods:L-carnitine(3.8 mM)was added to maturation medium and the effect was assessed in the quality(Experiment 1)and in the cleavage and 4-cells stage(Experiment 2).Besides,the effect of L-carnitine addition on maturation medium(3.8 mM)and culture medium(1.5 mM)on embryo rate production was assessed.In Experiment 1,bovine oocytes from abattoir were randomly separated into two groups(the control group and L-carnitine group)forin vitro maturation.Matured oocytes were examined for cumulus cells expansion as an indicator of maturation,and the content of the mitochondrial activity,the presence of lipid droplets,the reduced glutathione,and the reactive oxygen species were measured by using specific fluorochromes.In Experiment 2,oocytes were matured as performed in Experiment 1,afterward fertilized and cultured until day 3,and cleavage rate and 4-cells stage rate were determinated.In Experiment 3,in vitro maturation and fertilization were done as performed in Experiment 2,but at day 3 of culture,each group of embryos was separated into two new groups,and L-carnitine(1.5 mM)was added in culture media until day 8.The cleavage and embryo development rate were determined on the basis with the oocytes put on maturation.Hatching rate was calculated from cleaved embryos.Results:The cumulus expansion rate at gradeⅢand mitochondrial activity were significantly higher in the L-carnitine group in comparison with the control group(P0.05).In addition,cleavage and the proportion of embryo development and hatching rate were similar for all groups(P>0.05).Conclusions:L-carnitine as a supplement in culture media improves the cumulus expansion and increases the mitochondrial activity during in vitro maturation process but has no apparent effect on the cleavage and development of bovine embryos.Further investigations of L-carnitine addition on in vitroculture are needed to test their effect on embryo quality.

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《亚太生殖杂志（英文版）》 |2019年第6期|289-296|共8页
作者
Diego F. Carrillo-González; Nélida Rodríguez-Osorio; Charles R. Long; Neil A. Vásquez-Araque; Juan G. Maldonado-Estrada;
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One Health and Veterinary Innovative Research and Development Group School of Veterinary Medicine University of Antioquia 050034 Medellin Colombia;

Faculty of Agricultural Sciences School of Zootechnics Universidad de Sucre Colombia;

Genomics and Bioinformatics Lab Department of Biological Sciences University of the Republic of Uruguay Salto Campus Uruguay;

Department of Veterinary Physiology and Pharmacology College of Veterinary Medicine Texas A&M University College Station Texas 77843-4466 USA;

Grupo de investigación Biotecnología Animal Facultad de Ciencias Universidad Nacional de Colombia Sede Medellín Medellín Colombia;

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Effect of L-carnitine supplementation during in vitro maturation and in vitro culture on oocyte quality and embryonic development rate of bovines

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