Specific and cross-reactive immune response against Mycobacterium tuberculosis antigens in mice immunized with proteoliposomes from Mycobacterium bovis BCG

Nadine Alvarez; Reinaldo Acevedo; Luis Izquierdo; María Elena Sarmiento; Mohd-Nor Norazmi; José Luis Pérez; Armando Acosta; Daymí Serpa; Ramlah Kadir; Yanely Tirado; Reinier Borrero; Sonsire Fernández; Rubén Cabrera; Yolanda Valdes; Caridad Zayas

首页> 中文期刊> 《亚太热带生物医学杂志（英文版）》 >Specific and cross-reactive immune response against Mycobacterium tuberculosis antigens in mice immunized with proteoliposomes from Mycobacterium bovis BCG

Specific and cross-reactive immune response against Mycobacterium tuberculosis antigens in mice immunized with proteoliposomes from Mycobacterium bovis BCG

开具论文收录证明 >>

期刊封面封底目录下载 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Objective: To characterize the immunogenicity and the induction of cross-reactive re-sponses against Mycobacterium tuberculosis (M. tuberculosis) of a proteoliposome (PL) from Mycobacterium bovis Bacillus Calmette–Guérin (BCG) with and without alum hydroxide (AL) as adjuvant (PLBCG-AL and PLBCG, respectively) in BALB/c mice. Methods: BALB/c mice were inoculated with phosphate buffer solution, BCG, PLBCG and PLBCG-AL. The humoral immunogenicity was determined by ELISA [immuno-globulin G (IgG), IgG1 and IgG2a] and the cellular immunogenicity was evaluated in vivo by delayed type hypersensitivity. The humoral cross-reactive response against M. tuberculosis was determined by Western blot. Results: Sera from animals immunized with PLBCG-AL and PLBCG showed signifi-cant increase in specific total IgG and IgG1 antibodies and the presence of cross-reactive antibodies against M. tuberculosis antigens, which were more intense with the use of alum as adjuvant. Mice immunized with PLBCG and PLBCG-AL also showed a specific cellular response in vivo. Conclusions: The cellular and humoral immunogenicity of PLBCG and the capacity to induce cross-reactive responses against M. tuberculosis is in agreement with the pro-tective capacity previously demonstrated by this vaccine candidate and supports the continuation of its evaluation in further stages.

著录项

来源
《亚太热带生物医学杂志（英文版）》 |2017年第3期|188-192|共5页
作者
Nadine Alvarez; Reinaldo Acevedo; Luis Izquierdo; María Elena Sarmiento; Mohd-Nor Norazmi; José Luis Pérez; Armando Acosta; Daymí Serpa; Ramlah Kadir; Yanely Tirado; Reinier Borrero; Sonsire Fernández; Rubén Cabrera; Yolanda Valdes; Caridad Zayas;
展开▼
作者单位

Finlay Institute, Ave. 27, No. 19805, P.O. Box 16017, La Lisa, Havana CP11600, Cuba;

Finlay Institute, Ave. 27, No. 19805, P.O. Box 16017, La Lisa, Havana CP11600, Cuba;

Finlay Institute, Ave. 27, No. 19805, P.O. Box 16017, La Lisa, Havana CP11600, Cuba;

School of Health Sciences, University Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia;

School of Health Sciences, University Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia;

Institute for Research in Molecular Medicine, University Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia;

Finlay Institute, Ave. 27, No. 19805, P.O. Box 16017, La Lisa, Havana CP11600, Cuba;

Institute for Research in Molecular Medicine, University Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia;

Finlay Institute, Ave. 27, No. 19805, P.O. Box 16017, La Lisa, Havana CP11600, Cuba;

School of Health Sciences, University Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia;

Finlay Institute, Ave. 27, No. 19805, P.O. Box 16017, La Lisa, Havana CP11600, Cuba;

Finlay Institute, Ave. 27, No. 19805, P.O. Box 16017, La Lisa, Havana CP11600, Cuba;

Finlay Institute, Ave. 27, No. 19805, P.O. Box 16017, La Lisa, Havana CP11600, Cuba;

Finlay Institute, Ave. 27, No. 19805, P.O. Box 16017, La Lisa, Havana CP11600, Cuba;

Finlay Institute, Ave. 27, No. 19805, P.O. Box 16017, La Lisa, Havana CP11600, Cuba;

Finlay Institute, Ave. 27, No. 19805, P.O. Box 16017, La Lisa, Havana CP11600, Cuba;

展开▼
原文格式 PDF
正文语种 eng
中图分类
关键词

Specific and cross-reactive immune response against Mycobacterium tuberculosis antigens in mice immunized with proteoliposomes from Mycobacterium bovis BCG

摘要

著录项

相关主题

期刊订阅