Antibiotic susceptibility and molecular characterization of resistance genes among Escherichia coli and among Salmonella subsp. in chicken food chains

Yith Vuthy; Kruy Sun Lay; Heng Seiha; Alexandra Kerleguer; Awa Aidara-Kane

摘要

Objective: To investigate the occurrence of resistance genes among Escherichia coli (E. coli) and Salmonella subsp. isolated in chicken food chains in Phnom Penh, 2012–2013. Methods: Six hundred eighty two E. coli and 181 Salmonella Albany, Corvallis, and Kentucky strains were examined for susceptibilities to eight antimicrobials and following resistance genes were identified by PCR:blaTem, StrA, aadA, sul1, sul2, gyrA, Tet (A), and Tet (B). Results: E. coli presented high resistances to tetracycline, amoxicillin, and sulfameth-oxazole (63.1%–76.1%). Salmonella Albany and Salmonella Kentucky traduced high resistance percentages to amoxicillin, tetracycline, sulfamethoxazole, and nalidixic acid (84.6%–100%). Among amoxicillin-resistant isolates, blaTem genes were observed for 62%of E. coli isolates and 20%of 65 Salmonella Kentucky. The StrA gene was prevalent in 36%of 331 aminoglycoside-resistant E. coli and 90%of 40 aminoglycoside-resistant Salmonella Corvallis. The sul2 gene was predominant among sulfamethoxazole-resistant isolates, for 56%of 431 E. coli and 53%of 66 Salmonella Corvallis;the sul1 gene was observed in 54%of Salmonella Albany. The Tet (A) resistance gene was prevalent in E. coli (86%), Salmonella Corvallis (82%), Salmonella Kentucky (84%). High percentages of gyrA genes observed among nalidixic-acid resistant E. coli (91%), Salmonella Albany (92%), Salmonella Corvallis (75%) and Salmonella Kentucky (85%). Conclusions: Important occurrences of resistance gene were observed among E. coli and Salmonella in chicken food chains in Cambodia.

机译：目的：探讨大肠埃希菌和沙门氏菌亚种之间耐药基因的发生情况。 2012-2013年在金边的鸡肉食物链中分离得到。方法：检查了682株大肠杆菌和181株沙门氏菌奥尔巴尼，科瓦利斯和肯塔基州菌株对八种抗菌素的敏感性，并通过PCR鉴定了以下耐药基因：blaTem，StrA，aadA，sul1，sul2，gyrA，Tet（A ）和Tet（B）。结果：大肠杆菌对四环素，阿莫西林和磺胺甲基恶唑具有较高的耐药性（63.1％–76.1％）。沙门氏菌奥尔巴尼和肯塔基州沙门氏菌对阿莫西林，四环素，磺胺甲恶唑和萘啶酸的抗药性较高（84.6％–100％）。在耐阿莫西林的分离株中，发现62％的大肠杆菌分离株和65肯塔基州的沙门氏菌分离株的blaTem基因。 StrA基因在331个对氨基糖苷类耐药的大肠杆菌中占36％，在40个对氨基糖苷类耐药的沙门氏菌中占90％。在耐磺胺甲恶唑的菌株中，sul2基因占主导地位，占431大肠杆菌的56％和66克氏沙门氏菌的53％;在54％的沙门氏菌奥尔巴尼中观察到sul1基因。 Tet（A）抗性基因在大肠杆菌（86％），沙门氏菌Corvallis（82％），肯塔基州沙门氏菌（84％）中普遍存在。在耐萘啶酸的大肠杆菌（91％），沙门氏菌奥尔巴尼（92％），沙门氏菌科瓦利斯（75％）和肯塔基州沙门氏菌（85％）中观察到高百分比的gyrA基因。结论：柬埔寨鸡食物链中的大肠杆菌和沙门氏菌中发现了重要的抗性基因。

Antibiotic susceptibility and molecular characterization of resistance genes among Escherichia coli and among Salmonella subsp. in chicken food chains

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