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首页> 外文期刊>亚太热带生物医学杂志（英文版） >Antiviral activity of the dichloromethane extracts from Artocarpus heterophyllus leaves against hepatitis C virus
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Antiviral activity of the dichloromethane extracts from Artocarpus heterophyllus leaves against hepatitis C virus

机译：面包果叶中二氯甲烷提取物对丙型肝炎病毒的抗病毒活性

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摘要

Objective: To determine anti-viral activities of three Artocarpus species: Artocarpus altilis, Artocarpus camansi, and Artocarpus heterophyllus (A. heterophyllus) against Hepatitis C Virus (HCV). Methods: Antiviral activities of the crude extracts were examined by cell culture method using Huh7it-1 cells and HCV genotype 2a strain JFH1. The mode of action for anti-HCV activities was determined by time-of-addition experiments. The effect on HCV RNA replication and HCV accumulation in cells were analyzed by quantitative reverse transcription-PCR and western blotting, respectively. Results: The dichloromethane (DCM) extract of A. heterophyllus exhibited strong anti-HCV activity with an inhibitory concentration (IC50) value of (1.5 ± 0.6)μg/mL without obvious toxicity. The DCM extracts from Artocarpus altilis and Artocarpus camansi showed moderate anti-HCV activities with IC50 values being (6.5 ± 0.3) μg/mL and (9.7 ± 1.1) μg/mL, respectively. A time-of-addition studies showed that DCM extract from A. heterophyllus inhibited viral entry process though a direct virucidal activity and targeting host cells. HCV RNA replication and HCV protein expression were slightly reduced by the DCM treatment at high concentration. Conclusions: The DCM extract from A. heterophyllus is a good candidate to develop an antiviral agent to prevent HCV grant reinfection following liver transplantation.
机译：目的：确定三种面包果（Artocarpus altilis），面包果（Artocarpus camansi）和异果面包（A. heterophyllus）对丙型肝炎病毒（HCV）的抗病毒活性。方法：采用Huh7it-1细胞和HCV基因型2a菌株JFH1，通过细胞培养方法检测粗提物的抗病毒活性。抗HCV活性的作用方式是通过添加时间实验确定的。分别通过定量逆转录PCR和蛋白质印迹分析了对HCV RNA复制和HCV在细胞中积累的影响。结果：太子参二氯甲烷（DCM）提取物表现出较强的抗HCV活性，抑制浓度（IC50）值为（1.5±0.6）μg/ mL，无明显毒性。面包果和面包果的DCM提取物表现出中等的抗HCV活性，IC50值分别为（6.5±0.3）μg/ mL和（9.7±1.1）μg/ mL。加法时间研究表明，异叶农杆菌的DCM提取物通过直接的杀病毒活性和靶向宿主细胞抑制病毒进入过程。通过高浓度DCM处理，HCV RNA复制和HCV蛋白表达略有降低。结论：太子参中的DCM提取物是开发抗病毒剂以预防肝移植后HCV授予再感染的良好候选者。

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  • 来源
    《亚太热带生物医学杂志（英文版）》 |2017年第7期|633-639|共7页
  • 作者

    Achmad Fuad Hafid; Soetjipto; Hak Hotta; Chie Aoki-Utsubo; Adita Ayu Permanasari; Myrna Adianti; Lydia Tumewu; Aty Widyawaruyanti; Sri Puji Astuti Wahyuningsih; Tutik Sri Wahyuni; Maria Inge Lusida;

  • 作者单位

    Institute of Tropical Disease, Airlangga University, Surabaya 60115, Indonesia;

    Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Airlangga University, Surabaya 60286, Indonesia;

    Institute of Tropical Disease, Airlangga University, Surabaya 60115, Indonesia;

    Department of Biochemistry, Faculty of Medicine, Airlangga University, Surabaya 60131, Indonesia;

    Department of Oral Vaccine and Drug Development, Kobe University Graduate School of Health Sciences, 1-5-6 Minatojima-minamimachi, Chou-ku, Kobe 650-0047, Japan;

    Department of International Health, Kobe University Graduate School of Health Sciences, 7-10-2, Tomogaoka, Suma-ku, Kobe 654-0142, Japan;

    Institute of Tropical Disease, Airlangga University, Surabaya 60115, Indonesia;

    Institute of Tropical Disease, Airlangga University, Surabaya 60115, Indonesia;

    Institute of Tropical Disease, Airlangga University, Surabaya 60115, Indonesia;

    Institute of Tropical Disease, Airlangga University, Surabaya 60115, Indonesia;

    Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Airlangga University, Surabaya 60286, Indonesia;

    Department of Biology, Faculty of Science and Technology, Airlangga University, Surabaya 60115, Indonesia;

    Institute of Tropical Disease, Airlangga University, Surabaya 60115, Indonesia;

    Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Airlangga University, Surabaya 60286, Indonesia;

    Institute of Tropical Disease, Airlangga University, Surabaya 60115, Indonesia;

    Department of Microbiology, Faculty of Medicine, Airlangga University, Surabaya 60131, Indonesia;

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