An efficient method in breaking of dormancy from Bunium persicum (Boiss) Fedtsch seeds:a valuable herb of Middle East and Central Asia

Yousef Emamipoor; Mahmood Maziah

摘要

Objective: To develop a protocol for breaking of seed dormancy and increasing the seed germination rate of Bunium persicum. Methods:The seeds were treated with 3.1, 6.3, 12.5, 25, 50 and 100 µmol/L of benzyl aminopurine, gibberellic acid (GA3), thidiazuron (TDZ) and forchlorfenuron. Then, seeds were transferred to two different temperature conditions including room temperature (25 °C) and chilling temperature (2-5 °C). Results: The treatment of moist seeds with chilling temperature (2-5 °C) broke seed dormancy and showed maximum germination, which was 54.7%after 60 d treatment. Also, the treatment of dry seeds with chilling temperature broke seed dormancy with 9.3%germination rate after 120 d. Treatment of seeds with different level of plant growth regulators showed that under moist-room condition, there was evidence of higher and lower seed germination rate:GA3 (100 µmol/L) with 46.7%and TDZ (50 µmol/L) with 6.67%respectively. In addition, the results showed that under moist-chilling condition, TDZ (6.3 µmol/L) with 53.3%seed germination rate had higher influence on breaking seed dormancy. Treatment of seeds with combination of TDZ and GA3 under moist-chilling condition revealed higher rate of breaking of seed dormancy when 6.3 µmol/L TDZ was combined with 100 µmol/L GA3, showing 93.7%germination rate. Conclusions:The effect of plant growth regulators coupled with chilling temperature on breaking of seed dormancy could provide a large number of seedlings while the long juvenile time which is the next restricting factor of plantation still remained. Thus, the subsequent growth of seedlings to provide a large number of corms is necessary for successful plantation.

机译：目的：制定打破种子休眠，提高柿子种子发芽率的方案。方法：用3.1、6.3、12.5、25、50和100μmol/ L的苄基氨基嘌呤，赤霉素（GA3），噻二唑隆（TDZ）和甲草胺处理种子。然后，将种子转移到两个不同的温度条件下，包括室温（25°C）和冷却温度（2-5°C）。结果：在寒冷温度（2-5°C）下处理潮湿的种子打破了种子的休眠状态，并显示出最大的发芽率，处理60天后发芽率为54.7％。同样，在120 d后，用低温处理干燥种子破坏了种子休眠，发芽率为9.3％。用不同水平的植物生长调节剂处理种子表明，在潮湿环境下，种子发芽率有增有减的迹象：GA3（100 µmol / L）为46.7％，TDZ（50 µmol / L）为6.67％分别。此外，结果表明，在湿冷条件下，种子发芽率为53.3％的TDZ（6.3 µmol / L）对打破种子休眠有较大影响。将TDZ和GA3组合在湿冷条件下处理种子显示，当6.3 µmol / L TDZ与100 µmol / L GA3组合时，种子休眠的破坏率更高，发芽率为93.7％。结论：植物生长调节剂结合低温对打破种子休眠的影响可以提供大量的幼苗，而较长的幼年时间仍然是种植的下一个限制因素。因此，为了成功种植，随后的幼苗生长以提供大量的球茎是必要的。

An efficient method in breaking of dormancy from Bunium persicum (Boiss) Fedtsch seeds:a valuable herb of Middle East and Central Asia

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