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首页> 外文期刊>亚太热带生物医学杂志（英文版） >In vitro antioxidant, cytotoxic, thrombolytic activities and phytochemical evaluation of methanol extract of the A. philippense L. leaves
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In vitro antioxidant, cytotoxic, thrombolytic activities and phytochemical evaluation of methanol extract of the A. philippense L. leaves

机译：菲律宾落叶松甲醇提取物的体外抗氧化剂，细胞毒性，溶栓活性和植物化学评价

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摘要

To study the leaves of Adiantum philippense L. for their antioxidant, cytotoxicity and thrombolytic activities and to perform phytochemical evaluation. Methods: In-vitro antioxidant activity of extract was studied using DPPH radical scavenging, reducing power, total phenol and total flavonoid content determination assays. The cytotoxic activity was determined using brine shrimp lethality bioassay, thrombolytic activity by clot disruption and phytochemical potential by qualitative analysis. Results: The antioxidant activity of the extracts was found promising. The reducing power of this crude extract increase with the increase of concentration; IC50 values of DPPH scavenging activity was (140.00±0.86) µg/mL as compared to ascorbic acid [IC50 (130.00±0.76) µg/mL];Total phenol and total flavonoids content were (148.26±0.24) mg/mL and (163.06±0.56) mg/mL respectively. In cytotoxicity assay the LC50 values of the sample was (106.41±0.78) µg/mL where as for standard vincristin sulphate was (08.50±0.24) µg/mL as a positive control and the extract shows (12.86±1.02)% clot lytic whereas standard streptokinase shows (30.86±0.44% clot lytic activity in thrombolytic assay. The phytochemical evaluation indicates the presence of chemical constituents including carbohydrates, alkaloids, saponins, glycosides, flavonoids. Conclusions: This study shows that the methanol extract of leaves of Adiantum philippense L. has bioactivity but further compound isolation is necessary to confirm the activities of individual compounds.
机译：要研究铁线蕨的叶子的抗氧化剂，细胞毒性和溶栓活性，并进行植物化学评估。方法：采用DPPH自由基清除，还原能力，总酚和总黄酮含量测定法研究提取物的体外抗氧化活性。使用盐水虾致死性生物测定法测定细胞毒性活性，通过凝块破坏测定溶栓活性，通过定性分析测定植物化学潜力。结果：发现提取物的抗氧化活性很有希望。粗提物的还原能力随浓度的增加而增加;与抗坏血酸[IC50（130.00±0.76）µg / mL]相比，DPPH清除活性的IC50值为（140.00±0.86）µg / mL;总酚和总黄酮含量分别为（148.26±0.24）mg / mL和（163.06）分别为±0.56）mg / mL。在细胞毒性试验中，样品的LC50值为（106.41±0.78）µg / mL，而作为标准品，硫酸长春新素的标准品为（08.50±0.24）µg / mL，提取物的血凝块溶解度为（12.86±1.02）％，而标准链激酶在溶栓试验中显示出（30.86±0.44％的凝块溶解活性。植物化学评估表明存在化学成分，包括碳水化合物，生物碱，皂苷，糖苷，类黄酮。具有生物活性，但是进一步的化合物分离对于确认单个化合物的活性是必要的。

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