Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia

Gebreselema Gebreyohannes; Feleke Moges; Samuel Sahile; Nagappan Raja

首页> 外文期刊>亚太热带生物医学杂志（英文版） >Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia

【24h】

Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia

机译：从埃塞俄比亚塔纳湖的水和沉积物中分离和表征潜在的产生抗生素的放线菌

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

To isolate, evaluate and characterize potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia. Methods: A total of 31 strains of actinomycetes were isolated and tested against Gram positive and Gram negative bacterial strains by primary screening. In the primary screening, 11 promising isolates were identified and subjected to solid state and submerged state fermentation methods to produce crude extracts. The fermented biomass was extracted by organic solvent extraction method and tested against bacterial strains by disc and agar well diffusion methods. The isolates were characterized by using morphological, physiological and biochemical methods. Results: The result obtained from agar well diffusion method was better than disc diffusion method. The crude extract showed higher inhibition zone against Gram positive bacteria than Gram negative bacteria. One-way analysis of variance confirmed most of the crude extracts were statistically significant at 95% confidence interval. The minimum inhibitory concentration and minimum bactericidal concentration of crude extracts were 1.65 mg/mL and 3.30 mg/mL against Staphylococcus aureus, and 1.84 mg/mL and 3.80 mg/mL against Escherichia coli respectively. The growth of aerial and substrate mycelium varied in different culture media used. Most of the isolates were able to hydrolysis starch and urea; able to survive at 5% concentration of sodium chloride; optimum temperature for their growth was 30 °C. Conclusions: The results of the present study revealed that freshwater actinomycetes of Lake Tana appear to have immense potential as a source of antibacterial compounds.

机译：为了从埃塞俄比亚塔纳湖的水和沉积物中分离，评估和鉴定潜在的产生抗生素的放线菌。方法：分离出总共31株放线菌菌株，并通过初步筛选测试其对革兰氏阳性和革兰氏阴性细菌的影响。在初步筛选中，鉴定了11种有希望的分离物，并进行了固态和淹没式发酵方法生产粗提物。发酵后的生物质通过有机溶剂提取法提取，并通过圆盘和琼脂井扩散法对细菌菌株进行测试。通过使用形态学，生理学和生化方法对分离物进行表征。结果：琼脂孔扩散法得到的结果优于圆盘扩散法。粗提取物显示出比革兰氏阴性细菌更高的对革兰氏阳性细菌的抑制区。单向方差分析证实，大多数粗提物在95％置信区间内具有统计学意义。粗提物对金黄色葡萄球菌的最小抑菌浓度和最小杀菌浓度分别为1.65 mg / mL和3.30 mg / mL，对大肠杆菌的最小抑菌浓度为1.84 mg / mL和3.80 mg / mL。在所使用的不同培养基中，气生和底物菌丝体的生长变化。大多数分离物能够水解淀粉和尿素。能够在5％的氯化钠浓度下生存;它们生长的最佳温度为30°C。结论：本研究的结果表明，塔纳湖的淡水放线菌似乎具有作为抗菌化合物来源的巨大潜力。

著录项

来源
《亚太热带生物医学杂志（英文版）》 |2013年第6期|426-435|共10页
作者
Gebreselema Gebreyohannes; Feleke Moges; Samuel Sahile; Nagappan Raja;
展开▼
作者单位

Department of Biology, Faculty of Natural and Computational Sciences, Post Box 196, University of Gondar, Ethiopia;

Department of Medical Microbiology, College of Medical and Health Sciences, Post Box 196, University of Gondar, Ethiopia;

Department of Biology, Faculty of Natural and Computational Sciences, Post Box 196, University of Gondar, Ethiopia;

Department of Biology, Faculty of Natural and Computational Sciences, Post Box 196, University of Gondar, Ethiopia;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类
关键词

Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia

摘要

著录项

相关主题

期刊订阅