Effect of cytokinins on in vitro multiplication of Sophora tonkinensis

Sonali Jana; Iyyakkannu Sivanesan; Byoung Ryong Jeong

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Effect of cytokinins on in vitro multiplication of Sophora tonkinensis

机译：细胞分裂素对槐豆体外繁殖的影响

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Objective:To determine the effects of different cytokinins at various concentrations on in vitro shoot multiplication of an important medicinal plant. Methods: Nodal explants (1.5-2.0 cm) of Sophora tonkinensis were used. Multiple shoots were induced from nodal explants cultured on the Murashige and Skoog (MS) medium supplemented with 0.0, 0.5, 1.0, 2.0, 4.0, 8.0, or 16.0 µmol 2-isopentyladenine (2iP), N6 benzyladenine, kinetin or thiadiazuron. Results: Among the four investigated cytokinins, 2iP showed the best response for shoot multiplication. Maximum shoot induction (75%) was achieved on the MS medium supplemented with 2.0 µmol 2iP, with a mean number of 5.0 shoots per explant. In comparison to other cytokinins tried, 2iP showed the highest shoot elongation with a mean shoot length of 4.8 cm. Root initiation was observed within 15 d within the transfer of shoots onto the MS basal medium, and the rooting percentage was 100%with a mean number of 5.4 roots per shoot and root length of 6.2 cm over a period of 4 weeks. The healthy plants, hardened and transferred to a greenhouse for proper acclimatization, exhibited 100%survival. Conclusions:It can be summarized that 2iP is the optimal plant growth regulator for Sophora multiplication.

机译：目的：确定不同浓度的细胞分裂素对重要药用植物离体芽增殖的影响。方法：使用槐豆的节点外植体（1.5-2.0 cm）。从在补充有0.0、0.5、1.0、2.0、4.0、8.0或16.0 µmol 2-异opentyladenine（2iP），N6苄腺嘌呤，激动素或噻二唑的Murashige和Skoog（MS）培养基上培养的结节外植体诱导出多芽。结果：在四个调查的细胞分裂素中，2iP显示出最佳的芽增殖响应。在补充有2.0 µmol 2iP的MS培养基上达到最大芽诱导（75％），平均每个外植体有5.0个芽。与尝试的其他细胞分裂素相比，2iP表现出最高的枝条伸长率，平均枝条长度为4.8 cm。在将枝条转移至MS基础培养基上的15 d内观察到了根生，其生根率为100％，平均每根枝条有5.4根，在4周内根长为6.2 cm。健康的植物经过硬化并转移到温室中以使其适应环境，并具有100％的存活率。结论：2iP是槐豆繁殖的最佳植物生长调节剂。

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《亚太热带生物医学杂志（英文版）》 |2013年第7期|549-553|共5页
作者
Sonali Jana; Iyyakkannu Sivanesan; Byoung Ryong Jeong;
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Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, South Korea;

Institute of Agriculture&Life Science, Gyeongsang National University, Jinju 660-701, South Korea;

Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, South Korea;

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Effect of cytokinins on in vitro multiplication of Sophora tonkinensis

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