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首页> 外文期刊>亚太热带生物医学杂志（英文版） >Detection of class 1 integron in Acinetobacter baumannii isolates collected from nine hospitals in Turkey
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Detection of class 1 integron in Acinetobacter baumannii isolates collected from nine hospitals in Turkey

机译：从土耳其九家医院收集的鲍曼不动杆菌分离株中1类整合子的检测

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摘要

Objective: To investigate the antibiotic resistance genes inserted into class 1 and class 2 integrons in Acinetobacter baumannii (A. baumannii) isolates obtained from nine different cities in Turkey. Methods: A collection of 281 A. baumannii clinical isolates were collected from nine diferent state hospitals in Turkey and were confirmed as A. baumannii by conventional biochemical, API testing and bla-OXA-51 specific PCR. The isolates were examined by PCR for existence of class 1 and 2 integron gene cassettes. Results: They were characterized by antimicrobial susceptibility testing and the highest resistance rates were determined for piperacillin (90.03%), ciprofloxacin (87.54%), cefepime and trimethoprim/sulfamethoxazole (81.13%). The lowest resistance rates was for cefotaxime (3.55%). class I integrons were detected in 6.4%(18/281) of A. baumannii strains and no class 2 integron was detected. The gene cassettes of class 1 integrons AacC1-AAC(3)I-aadA1, AacC1-aadA1, AAC(3)-I, AAC(3)-I-AAC(3)-I-aadA1, TEM-1, AAC(3)-I-aadA1-AAC(3)-I-AAC(3)-I, AAC(3)-I-AAC(3)-I-AAC(3)-I-aadA1, AAC(3)-I-aadA1, AAC(3)-I-AAC(3)-I, AAC(3)-I-aadA1- AAC(3)-I-aadA1, AAC(3)-I- AAC(3)-I-aadA1-AAC(3)-I-aadA1 were detected in eighteen strains. The aac genes family were most frequently found integrated into the class 1 integrons and it was followed by aadA genes and TEM-1 genes. Conclusions: This is an extensive study on the distribution of class 1 integron among A. baumannii in Turkey. In addition to these, two new alleles were observed. Their percentage rates of similarity to other cassettes are 95%aadA1 ( TKA18) and 89%aadA1 (ANKA3).
机译：目的：研究从土耳其九个不同城市获得的鲍曼不动杆菌（A. baumannii）分离株中插入1类和2类整合素的抗生素抗性基因。 方法：从土耳其的9家州立医院中收集了281株鲍曼不动杆菌临床分离株，并通过常规生化，API测试和bla-OXA-51特异性PCR确认为鲍曼不动杆菌。通过PCR检查分离物是否存在1类和2类整合子基因盒。 结果：通过抗菌药敏试验对它们进行了表征，并确定了哌拉西林（90.03％），环丙沙星（87.54％），头孢吡肟和甲氧苄啶/磺胺甲恶唑（81.13％）的最高耐药率。头孢噻肟的耐药率最低（3.55％）。在6.4％（18/281）的鲍曼不动杆菌菌株中检​​测到I类整合素，未检测到2类整合子。 1类整合素的基因盒AacC1-AAC（3）I-aadA1，AacC1-aadA1，AAC（3）-I，AAC（3）-I-AAC（3）-I-aadA1，TEM-1，AAC（ 3）-I-aadA1-AAC（3）-I-AAC（3）-I，AAC（3）-I-AAC（3）-I-AAC（3）-I-aadA1，AAC（3）-I -aadA1，AAC（3）-I-AAC（3）-I，AAC（3）-I-aadA1- AAC（3）-I-aadA1，AAC（3）-I- AAC（3）-I-aadA1在十八个菌株中检测到-AAC（3）-I-aadA1。最常发现aac基因家族整合到1类整合素中，其次是aadA基因和TEM-1基因。 结论：这是对土耳其鲍氏不动杆菌中1类整合子分布的广泛研究。除了这些，还观察到两个新的等位基因。它们与其他盒的相似性百分比为95％aadA1（TKA18）和89％aadA1（ANKA3）。

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  • 来源
    《亚太热带生物医学杂志（英文版）》 |2013年第9期|743-747|共5页
  • 作者

    Ayşegül Çopur ÇİÇek; Yasemin Ay Alıtntop; Ahmet Çalışkan; Yelda Yazıcı; Cemal Sandallı; Azer Özad Düzgün; Ayşegül Saral; Tuba Kayman; Zeynep Çİzmecİ; Pervin Özlem Balcı; Tuba Dal; Mehmet Fırat; İsmail Tosun;

  • 作者单位

    Department of Medical Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey;

    General State Hospital, Niğde, Turkey;

    Microbiology Laboratory, Necip Fazıl City Hospital, Kahramanmaraş, Turkey;

    Clinical Microbiology Laboratory, Ahi Evren Chest and Cardiovascular Surgery Research and Training Hospital, Trabzon, Turkey;

    Department of Biology, Faculty of Arts and Sciences, Recep Tayyip Erdogan University, Rize, Turkey;

    Department of Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Gümüşhane University, Gümüşhane, Turkey;

    Department of Biology, Faculty of Arts and Sciences, Artvin Coruh University, Artvin, Turkey;

    Clinical Microbiology Laboratory, Research and Training Hospital, Kayseri, Turkey;

    Clinical Microbiology Laboratory, Kecioren Research and Training Hospital, Ankara, Turkey;

    Clinical Microbiology Laboratory, General State Hospital, Tokat, Turkey;

    Department of Medical Microbiology, Faculty of Medicine, Dicle University, Diyarbakir, Turkey;

    Clinic of Infectious Diseases and Clinical Microbiology, Private 0SM 0rtadogu Hospital, Sanliurfa, Turkey;

    Microbiology Laboratory, Kırıkkale Highly Specialized Hospital, Kırıkkale, Turkey;

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