Does Carica papaya leaf-extract increase the platelet count? An experimental study in a murine model

Susiji Wickramasinghe; Roshitha Nilmini Waduge

摘要

Objective:To investigate the potential role of fresh Carica papaya (C. papaya) leaf extract on haematological and biochemical parameters and toxicological changes in a murine model. Methods: In total 36 mice were used for the trial. Fresh C. papaya leaf extract [0.2 mL (2 g)/mouse] was given only to the test group (18 mice). General behavior, clinical signs and feeding patterns were recorded. Blood and tissue samples were collected at intervals. Haematological parameters including platelet, red blood cell (RBC), white blood cell (WBC), packed cell volume (PCV), serum biochemistry including serum creatinine, serum glutamic-oxaloacetic transaminase (SGOT) and serum glutamic-pyruvic transaminase (SGPT) were determined. Organs for possible histopathological changes were examined. Results: Neither group exhibited alteration of behavior or reduction in food and water intake. Similarly, no significant changes in SGOT, SGPT and serum creatinine levels were detected in the test group. Histopathological organ changes were not observed in either group of mice except in three liver samples of the test group which had a mild focal necrosis. The platelet count (11.33±0.35)í105/µL (P=0.000 04) and the RBC count (7.97±0.61)í106/µL (P=0.000 03) were significantly increased in the test group compared to that of the controls. However, WBC count and PCV (%) values were not changed significantly in the test group. The platelet count in the test group started to increase significantly from Day 3 (3.4±0.18í105/µL), reaching almost a fourfold higher at Day 21 (11.3í105/µL), while it was 3.8í105/µL and 5.5í105/µL at Day 3 and Day 21 respectively in the control. Likewise, the RBC count in the test group increased from 6í106/µL to 9í106/ µL at Day 21 while it remained near constant in the control group (6í106/µL). Conclusions: Fresh C. papaya leaf extract significantly increased the platelet and RBC counts in the test group as compared to controls. Therefore, it is very important to identify those chemicals of C. papaya leaves as it can be recommended to be used as a medication to boost thrombopoiesis and erythropoiesis in humans and in animals in which these cell lineages have been compromised.

机译：目的：研究新鲜番木瓜叶提取物对小鼠模型血液学和生化指标及毒理学变化的潜在作用。方法：总共使用了36只小鼠进行试验。新鲜的番木瓜叶提取物[0.2 mL（2 g）/小鼠]仅给予测试组（18只小鼠）。记录一般行为，临床体征和喂养方式。定期采集血液和组织样本。血液学参数包括血小板，红细胞（RBC），白细胞（WBC），堆积细胞体积（PCV），包括血清肌酐的血清生化，血清谷草-草酰乙酸转氨酶（SGOT）和血清谷氨酸-丙酮酸转氨酶（SGPT）。决心。检查可能的组织病理学改变的器官。结果：两组均未表现出行为改变或食物和水摄入量减少。同样，在测试组中未发现SGOT，SGPT和血清肌酐水平有明显变化。在两组小鼠中均未观察到组织病理学器官变化，除了在试验组的三个肝脏样品中有轻度局灶性坏死。与对照组相比，试验组的血小板计数（11.33±0.35）×105 /μL（P = 0.000 04）和RBC计数（7.97±0.61）×106 /μL（P = 0.000 03）显着增加。但是，在测试组中，WBC计数和PCV（％）值没有显着变化。从第3天起，测试组的血小板计数开始显着增加（3.4±0.18×105 / µL），在第21天达到近四倍（11.3×105 / µL），分别为3.8×105 / µL和5.5×105 / µL。分别在对照组的第3天和第21天。同样，在第21天，测试组的RBC计数从6×106 / µL增加到9×106 / µL，而对照组的红细胞计数则保持接近恒定（6×106 / µL）。结论：与对照组相比，试验组新鲜番木瓜叶提取物显着增加了血小板和RBC计数。因此，鉴定番木瓜叶中的那些化学物质非常重要，因为可以建议将其用作增强人类和这些细胞谱系受损的动物的血小板生成和红细胞生成的药物。

Does Carica papaya leaf-extract increase the platelet count? An experimental study in a murine model

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