Bioefficacy of crude extract of Cyperus aromaticus (Family：Cyperaceae) cultured cells, against Aedes aegypti and Aedes albopictus mosquitoes

Fatemeh Kamiabi; Zairi Jaal; Chan Lai Keng

摘要

Objective:To evaluate the growth inhibition activity of the crude extract of Cyperus aromaticus (C. aromaticus) cultured cells against the 3rd instar larvae of Aedes aegypti (Linn.) and Aedes albopictus Skuse (Ae. albopictus) under laboratory conditions, and determine the sublethal effects (EI50) of the crude extract of C. aromaticus cultured cells on some biological and morphological parameters of both Aedes mosquito species during two generations as well. Methods:The cell suspension cultures of C. aromaticus were activated from five callus lines (P4, Pa, Z1, Z6 and Ml) derived from the root explants of in vitro plantlets. The cultured cells were extracted in chloroform and used as plant material for the present study. For detection of juvenile hormone III, the crude extracts were analyzed by HPLC. Then the crude extracts of the three C. aromaticus cultured cell lines which contained varied amounts of juvenile hormone III [high level (P4 cell line), medium level (Z1 cell line) and low level (Ml cell line)] were tested against Aedes mosquito species. Laboratory evaluation was performed against late third instar larvae of the Vector Control Research Unit strains of Ae. aegypti and Ae. albopictus using the standard WHO method. The effects of EI50 of the C. aromaticus cultured P4 cells on fecundity, fertility, growth period, sex ratio, adult size and longevity of Aedes mosquitoes were assessed. Results:Bioassay tests presented the remarkable growth inhibition activity of the crude extracts of C. aromaticus cultured cells against the two Aedes mosquitoes. Between the two mosquito species, Ae. albopictus was more susceptible to the crude extracts with lower EI50 values. EI50 of the crude extract of C. aromaticus cultured cells (P4) increased the sterility indices in the parental generation females in both Aedes mosquito species. A significant delay in the pupal formation and adult emergence were observed in the parental generation of the both mosquito species. The sex ratio of the adult population either parental or F1 generation of the Aedes mosquito species was not significantly affected by the EI50 dosage of the crude extract of C. aromaticus cultured P4 cells. A significant decrease in the wing length of the treated adult (female and male) of Aedes aegypti as well as the treated female of Ae. albopictus were observed. Longevity of the adult female of the parental generation of both Aedes mosquitoes as well as females of F1 generation of Ae. albopictus were significantly decreased. Conclusions: The present study revealed the potential of the crude extract of C. aromaticus cultured cells in controlling vector mosquito populations in the effort to reduce the transmission of vector borne diseases.

机译：目的：在实验室条件下评价香附子粗提物对埃及伊蚊和白纹伊蚊三龄幼虫的生长抑制活性，并测定其抑菌活性。香椿菌培养细胞粗提物的亚致死效应（EI50）对两代伊蚊的某些生物学和形态学参数的影响。方法：从5个愈伤组织（P4，Pa，Z1，Z6和M1）中提取芳香环衣藻的细胞悬浮培养物，这些愈伤组织来自试管苗的根外植体。将培养的细胞在氯仿中提取，并用作本研究的植物材料。为了检测少年激素III，通过HPLC分析粗提取物。然后，针对伊蚊测试了三种含有不同量的少年激素III [高水平（P4细胞系），中水平（Z1细胞系）和低水平（M1细胞系）]的芳香族梭菌培养细胞系的粗提物。蚊子种类。针对Ae的Vector Control Research Unit菌株的第三龄幼虫进行了实验室评估。埃及和埃及。使用标准WHO方法的白化病。评估了C.aromaticus培养的P4细胞的EI50对伊蚊的繁殖力，繁殖力，生长期，性别比，成年大小和寿命的影响。结果：生物测定法测试结果显示，C.aromaticus培养细胞的粗提物对两种伊蚊具有显着的生长抑制活性。在两种蚊子之间，Ae。白皮病更容易受到EI50值较低的粗提物的影响。 C.aromaticus培养细胞（P4）的粗提物的EI50增加了两种伊蚊的父母代雌性的不育指数。在这两种蚊子的亲代中，observed的形成和成年的出现都明显延迟。蚊的亲本或F1代成年种群的性别比例不受芳香族梭状芽胞杆菌培养的P4细胞粗提物的EI50剂量的显着影响。受治疗的埃及伊蚊成年（雌性和雄性）以及受治疗的雌成年雌性的机翼长度显着减少。观察到白化病。伊蚊的父母代和成年雌性的F1代的成年女性的寿命。白带明显减少。结论：本研究揭示了芳香丙酸梭菌培养细胞粗提物在控制媒介蚊子种群中的潜力，以减少媒介传播疾病的传播。

Bioefficacy of crude extract of Cyperus aromaticus (Family：Cyperaceae) cultured cells, against Aedes aegypti and Aedes albopictus mosquitoes

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