Evaluation of cytotoxic and anti-tumor activity of partially purified serine protease isolate from the Indian earthworm Pheretima posthuma

Mahendra Kumar Verma; Francies Xavier; Yogendra Kumar Verma; Kota Sobha

首页> 外文期刊>亚太热带生物医学杂志（英文版） >Evaluation of cytotoxic and anti-tumor activity of partially purified serine protease isolate from the Indian earthworm Pheretima posthuma

【24h】

Evaluation of cytotoxic and anti-tumor activity of partially purified serine protease isolate from the Indian earthworm Pheretima posthuma

机译：印度earthPheretima posthuma部分纯化的丝氨酸蛋白酶分离物的细胞毒性和抗肿瘤活性的评估

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Objective: To isolate, partially purify and evaluate the cytotoxic and antitumor activity of a serine protease from the chosen Indian earthworm Pheretima posthuma.Methods:Whole animal extract was prepared and purified its protein constituents by size and charge based chromatographic separation techniques using Sephadex G-50 and DEAE-Cellulose resin respectively. Average molecular weight of the protein isolate was determined and analyzed for its cytotoxic property against Vero cells in different dilutions (1: 20 and 1: 40) and anti-tumor activity by MTT assay (a colorimetric assay) using breast cancer cell line MCF-7, with tamoxifen as standard.Results:One of the protein constituents after purification was characterized as serine protease by Caseinolytic plate diffusion assay. Average molecular weight of this purified isolate was determined, by SDS-PAGE analysis with standard protein ladder, as of 15 kDa. The performed tests suggested that the 15kDa fraction has potent cytotoxic activity and satisfactory antitumor activity as well in vitro.Conclusions:Exact molecular mechanism of the cytotoxic and antitumor activities is yet to be explored and currently we are working on ultra-purification and biophysical characterization of this fraction. Further investigation into the mechanism(s) of cytotoxic and antitumor activities at molecular level would be useful in treatment of various classes of cancer and viral infections in future.

机译：目的：从所选印度earthPheretima posthuma中分离，部分纯化和评估丝氨酸蛋白酶的细胞毒性和抗肿瘤活性。方法：使用Sephadex G-通过基于大小和电荷的色谱分离技术，制备全动物提取物并纯化其蛋白质成分。 50和DEAE-纤维素树脂。确定蛋白质分离物的平均分子量，并使用乳腺癌细胞系MCF-通过MTT分析（比色分析）分析其在不同稀释度（1:20和1:40）中对Vero细胞的细胞毒性，以及抗肿瘤活性。 7，以他莫昔芬为标准。结果：通过酪蛋白水解平板扩散分析将纯化后的一种蛋白质成分表征为丝氨酸蛋白酶。通过用标准蛋白梯的SDS-PAGE分析测定该纯化分离物的平均分子量，为15kDa。进行的实验表明，15kDa的部分具有强大的细胞毒活性和令人满意的抗肿瘤活性。结论：细胞毒和抗肿瘤活性的确切分子机制尚待探索，目前我们正在进行超纯化和生物物理表征。这个分数。在分子水平上进一步研究细胞毒性和抗肿瘤活性的机制将对将来治疗各种癌症和病毒感染有用。

著录项

来源
《亚太热带生物医学杂志（英文版）》 |2013年第11期|896-901|共6页
作者
Mahendra Kumar Verma; Francies Xavier; Yogendra Kumar Verma; Kota Sobha;
展开▼
作者单位

Department of Biotechnology, R.V.R. & J.C. College of Engineering, Guntur, 522 019, Andhra Pradesh, India;

Department of Biotechnology, R.V.R. & J.C. College of Engineering, Guntur, 522 019, Andhra Pradesh, India;

Department of Microbiology, All India Institute of Medical Sciences, New Delhi,110 029, India;

Department of Biotechnology, R.V.R. & J.C. College of Engineering, Guntur, 522 019, Andhra Pradesh, India;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类
关键词

Evaluation of cytotoxic and anti-tumor activity of partially purified serine protease isolate from the Indian earthworm Pheretima posthuma

摘要

著录项

相关主题

期刊订阅