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首页> 外文期刊>亚太热带生物医学杂志（英文版） >Combination of low-concentration of novel phytoestrogen (8,9)-furanyl-pterocarpan-3-ol from Pachyrhizus erosus attenuated tamoxifen-associated growth inhibition on breast cancer T47D cells
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Combination of low-concentration of novel phytoestrogen (8,9)-furanyl-pterocarpan-3-ol from Pachyrhizus erosus attenuated tamoxifen-associated growth inhibition on breast cancer T47D cells

机译：低浓度新型Pachyrhizus eros的植物雌激素（8,9）-呋喃基-呋喃丹-3-醇的组合减弱了他莫昔芬相关的乳腺癌T47D细胞生长抑制作用

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Objective: To investigate the estrogenic effect of (8,9)-furanyl-pterocarpan-3-ol (FPC) on growth of human breast cancer T47D cells and the interactions between the FPC and tamoxifen (TAM), on the growth of estrogen receptor-dependent breast cancer T47D cells.Methods:T47D cells were treated with FPC alone (0.01-200 μmol/L) or in combination with TAM 20 nmol/L. The proliferation effect of FPC were conducted on T47D cells in vitro by MTT test. Furthermore, the expression of ERα or c-Myc were also determined by immunohistochemistry.Results:inhibitory effect on T47D cells, wheraes co-administered with low concentration (less than 1μmol/L) of FPC attenuated to promote cell proliferation. In contrast, the combination of TAM with higher doses (more than 20 μmol/L) of FPC showed growth inhibitory. This result was supported by immunocytochemistry studies that the administration of 20 nmol/L TAM down-regulated ER-αand c-Myc, but the combination of 20 nmol/L TAM and 1 μmol/L FPC robustly up-regulated expression of ER-α. Thus, the reduced growth inhibition of TAM 20 nmol/L by FPC 1 μmol/L on T47D cells may act via the modulation of ER-α.Conclusions:The findings indicate and suggest that FPC had estrogenic activity at low The results indicated that administration of an anti-estrogen TAM showed growth concentrations and anti-estrogenic effect that are likely to be regulated by c-Myc and estrogen receptors. We also confirm that low concentration of FPC attenuated the growth-inhibitory effects of TAM on mammary tumor prevention. Therefore, the present study suggests that caution is warranted regarding the consumption of dietary FPC by breast cancer patients while on TMA therapy.
机译：目的：研究（8,9）-呋喃基-呋喃丹-3-醇（FPC）对人乳腺癌T47D细胞生长的雌激素作用以及FPC与他莫昔芬（TAM）的相互作用对雌激素受体生长的影响方法：将T47D细胞单独用FPC（0.01-200μmol/ L）或与TAM 20 nmol / L联合处理。 FTT法通过MTT法在体外对T47D细胞进行增殖。结果：免疫组化研究发现，低浓度（小于1μmol/ L）的FPC共同作用于小鼠T47D细胞具有抑制作用，并能促进细胞增殖。相反，TAM与更高剂量（大于20μmol/ L）的FPC的组合显示出生长抑制作用。免疫细胞化学研究支持这一结果，即20 nmol / L TAM的给药下调了ER-α和c-Myc，但20 nmol / L TAM和1μmol/ L FPC的结合却强烈上调了ER-α的表达。因此，FPC 1μmol/ L降低TAM 20 nmol / L对T47D细胞的生长抑制作用可能是通过调节ER-α起作用的。结论：研究结果表明FPC在较低的温度下具有雌激素活性。抗雌激素TAM的剂量显示其生长浓度和抗雌激素作用可能受c-Myc和雌激素受体调节。我们还证实低浓度的FPC减弱了TAM对预防乳腺肿瘤的生长抑制作用。因此，本研究表明，在接受TMA治疗的乳腺癌患者中应注意饮食中FPC的摄入。

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  • 来源
    《亚太热带生物医学杂志（英文版）》 |2013年第11期|847-852|共6页
  • 作者

    Arief Nurrochmad; Endang Lukitaningsih; Ameilinda Monikawati; Dita Brenna Septhea; Edy Meiyanto;

  • 作者单位

    Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta-55281, Indonesia;

    Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281, Indonesia;

    Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281, Indonesia;

    Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281, Indonesia;

    Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281, Indonesia;

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