In vitro analysis on bactericidal screening and antioxidant potentiality of leaf and root extracts of Thottea siliquosa (Lam.) Ding Hou. An ethnobotanical plant

Saiba Abdul Wahab Nusaiba; Kumaraswamy Murugan

摘要

Objective: Natural products of plant origin are potential source of novel antimicrobial and antioxidative agents. Thottea siliquosa (Lam.) Ding Hou. (T. siliquosa). A medicinal herb used by local tribals for treating various ailments. The present study aims at the phytochemical screening, GC-MS analysis, in vitro antibacterial activity and antioxidant potentiality of root and leaf extracts of T. siliquosa.Methods:Hot continuous Soxhlet extraction, GC-MS analysis, antibacterial analysis by disc diffusion, microdilution assay and antioxidant potentialities by hydroxyl radical and nitric oxide radical scavenging. The data was statistically analyzed. Results: Phytochemical screening of the ethyl acetate and methanolic extract of leaf and root revealed the presence of phenols, alkaloids, tannins and saponin. The extract revealed a pool of phytochemicals by comparison with authentic standards from spectral library. Both the extracts has shown their broad spectrum of inhibition against the selected bacteria Staphylococcus aureus,Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia compared with standard antibiotic drug streptomycin. The extracts showed antioxidant activity by scavenging of free radicals such as hydroxyl and nitric oxide. The IC50 values of the ethyl acetate extracts leaf and root and standard in this assay were 167.5±0.67, 99.4±1.2, 192±2.5 µg/mL respectively. Similarly those methanolic extracts of leaf and root were 269.5±0.89 and 289.1±2.66 µg/mL respectively. Similarly, ethyl acetate and methanolic extracts also caused a moderate dose-dependent inhibition of nitric oxide with an IC50 range 65.5±1.55 to 148 ±3.09 µg/mL. The inhibitory activities were found to be dose dependent.Conclusion:The present study provides evidence that ethyl acetate and methanol extract of leaf and root of T. siliquosa are potential source of natural antioxidants and bactericidal nature. It is essential that research should continue to isolate and purify the bio active components of this natural plant and use in drug discovery and development.

机译：目的：植物来源的天然产物是新型抗微生物剂和抗氧化剂的潜在来源。 Thottea siliquosa（Lam。）丁厚。（T. siliquosa）。当地部落使用的一种药草，用于治疗各种疾病。本研究主要针对刺槐的根，叶提取物进行植物化学筛选，GC-MS分析，体外抑菌活性和抗氧化能力。方法：热连续索氏提取，GC-MS分析，圆盘扩散抗菌分析，微稀释羟基自由基和一氧化氮自由基的清除测定和抗氧化剂的潜力。对数据进行统计分析。结果：对叶和根的乙酸乙酯和甲醇提取物进行植物化学筛选后发现，其中存在酚，生物碱，单宁和皂苷。通过与光谱库中的真实标准品进行比较，该提取物揭示出一批植物化学物质。与标准抗生素药物链霉素相比，两种提取物均显示出对所选细菌金黄色葡萄球菌，大肠杆菌，铜绿假单胞菌和肺炎克雷伯菌的广泛抑制作用。提取物通过清除自由基（例如羟基和一氧化氮）显示出抗氧化活性。本试验中乙酸乙酯提取物的叶，根和标准品的IC50值分别为167.5±0.67、99.4±1.2、192±2.5 µg / mL。同样，叶和根的甲醇提取物分别为269.5±0.89和289.1±2.66 µg / mL。同样，乙酸乙酯和甲醇提取物也引起对一氧化氮的中等剂量依赖性抑制，IC50范围为65.5±1.55至148±3.09 µg / mL。结论：本研究提供了证据，证明刺槐叶和根的乙酸乙酯和甲醇提取物是天然抗氧化剂和杀菌性质的潜在来源。至关重要的是，研究应继续分离和纯化该天然植物的生物活性成分，并将其用于药物发现和开发。

In vitro analysis on bactericidal screening and antioxidant potentiality of leaf and root extracts of Thottea siliquosa (Lam.) Ding Hou. An ethnobotanical plant

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