Phytochemical investigation and in vitro antioxidant activity of an indigenous medicinal plant Alpinia nigra B.L. Burtt

Suprava Sahoo; Goutam Ghosh; Debajyoti Das; Sanghamitra Nayak

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Phytochemical investigation and in vitro antioxidant activity of an indigenous medicinal plant Alpinia nigra B.L. Burtt

机译：本地药用植物黑羽Alpinia nigra B.L.的植物化学研究和体外抗氧化活性伯特

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Objective: To investigate antioxidant potential of methanol extract of Alpinia nigra leaves.Methods:picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), nitric oxide and hydrogen peroxide radical scavenging assays. Phytochemical constituents, total phenolic content and total flavonoid content of the extract at different concentrations (10-500 µg/mL) were determined.Results: Alpinia nigra leaves showed high free radical scavenging activity as evidenced by the low IC50 values in DPPH (64.51 µg/mL), in ABTS (28.32 µg/mL), in nitric oxide (80.02 µg/mL) and in H2O2 (77.45 µg/mL) scavenging assays. Furthermore the TPC and TFC of the extract were found to be 69.25 mg gallic acid equivalent per gram of extract and 78.84 mg quercetin equivalent per gram of extract respectively.Conclusions:The results of present comprehensive analysis demonstrated that Alpinia nigra The study was done by using various in vitro methods such as 1,1-diphenyl-2-leaves possess high phenolic, flavonoid contents and potential antioxidant activity, and could be used as a viable source of natural antioxidants and might be exploited for functional foods and neutraceutical applications.

机译：目的：研究黑加仑叶甲醇提取物的抗氧化能力。方法：苦瓜酰肼（DPPH），2，2-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）（ABTS），一氧化氮和过氧化氢自由基清除法。测定了不同浓度（10-500 µg / mL）提取物的植物化学成分，总酚含量和总黄酮含量。结果：黑刺槐叶片显示出较高的自由基清除活性，这在DPPH中的IC50值较低（64.51 µg）证明了这一点。 / mL），ABTS（28.32 µg / mL），一氧化氮（80.02 µg / mL）和H2O2（77.45 µg / mL）清除试验。此外，提取物的TPC和TFC分别为每克提取物69.25 mg没食子酸当量和每克提取物78.84 mg槲皮素当量。结论：目前的综合分析结果表明，黑刺槐通过使用1,1-二苯基-2-叶等多种体外方法具有较高的酚类，类黄酮含量和潜在的抗氧化剂活性，可以用作天然抗氧化剂的可行来源，并可以用于功能性食品和中性营养保健用途。

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《亚太热带生物医学杂志（英文版）》 |2013年第11期|871-876|共6页
作者
Suprava Sahoo; Goutam Ghosh; Debajyoti Das; Sanghamitra Nayak;
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Centre of Biotechnology, Siksha 0 Anusandhan University, Bhubaneswar, 0disha-751030, India;

School of Pharmaceutical Sciences, Siksha 0 Anusandhan University, Bhubaneswar, 0disha-751030, India;

School of Pharmaceutical Sciences, Siksha 0 Anusandhan University, Bhubaneswar, 0disha-751030, India;

Centre of Biotechnology, Siksha 0 Anusandhan University, Bhubaneswar, 0disha-751030, India;

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Phytochemical investigation and in vitro antioxidant activity of an indigenous medicinal plant Alpinia nigra B.L. Burtt

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