Study on the antiulcer effects of Veronicastrum axillare on gastric ulcer in rats induced by ethanol based on tumor necrosis factor-α (TNF-α) and endothelin-1 (ET-1)

Yong Du; Weichun Zhao; Leilei Lu; Jiayan Zheng; Xishi Hu; Zhehan Yu; Lixin Zhu

摘要

Objective:To assess whether Veronicastrum axillare (V. axillare) can ameliorate ethanol-induced gastric mucosal lesions in rats, reduce the production of pro-inflammatory cytokines, suppress apoptosis and improve local microcirculation disturbances. Methods:Totally 48 male Sprague-Dawley rats were randomly divided into six groups, eight rats in each group. Rats in the normal group and the model group were administered with 0.9%normal saline respectively. Rats in the positive group and ranitidine group were administered with 0.18% ranitidine suspension by intragastric administration respectively. Those in the high dose V. axillare group, the medium dose V. axillare group and the low dose V. axillare group were administrated with V. axillare at the daily dose of 2.8 g/kg, 1.4 g/kg and 0.7 g/kg by intragastric administration. Gastric mucosal lesions were produced by intragastric administration of absolute ethanol. Water extract of V. axillare was successively injected for 14 d and last day was injected 1 h before ethanol administration. Gastric mucosal ulcer index and ulcer inhibitory rate were counted by improved Guth methods. The tissue sections were made for pathological histology analysis. Also, we measured the concentrations of tumor necrosis factor-α (TNF-α) and endothelin-1 (ET-1) in gastric mucosal, as an index of the pro-inflammatory cytokines, apoptosis and local microcirculation. Besides, the mRNA contents of TNF-αand ET-1 were measured to verify effects on gene expression by real-time fluorescent quantitative PCR. Results: Water extract of V. axillare significantly ameliorated the gastric mucosal lesions induced by ethanol administration (P<0.01). Pro-inflammatory cytokines, TNF-αand ET-1 were increased after ethanol administration and significantly reduced by water extract of V. axillare. The expressions of TNF-αand ET-1 mRNA were also be inhibited by water extract of V. axillare. Conclusion: Current evidences show water extract of V. axillare is effective for defending against ethanol-induced gastric mucosal lesions, significantly inhibiting the production of pro-inflammatory cytokines and the expressions of TNF-αand ET-1 mRNA, which may be useful for inhibiting apoptosis and improving local microcirculation.

机译：目的：评估腋臭Veronicastrum（V. axillare）是否可以改善乙醇诱发的大鼠胃粘膜损伤，减少促炎细胞因子的产生，抑制细胞凋亡并改善局部微循环障碍。方法：总共48只雄性Sprague-Dawley大鼠随机分为六组，每组八只。正常组和模型组分别给予0.9％生理盐水。阳性组和雷尼替丁组分别通过胃内给药给予0.18％雷尼替丁混悬液。高剂量腋臭腋下组，中等剂量腋臭组和低剂量腋臭组分别以每日2.8 g / kg，1.4 g / kg和0.7 g / kg的剂量服用腋臭通过胃内给药。通过无水乙醇的胃内给药产生胃粘膜损伤。连续注射腋臭水提取物14天，最后一天在施用乙醇前1小时注射。胃黏膜溃疡指数和溃疡抑制率采用改良的Guth方法计算。制作组织切片用于病理组织学分析。此外，我们测量了胃黏膜中肿瘤坏死因子-α（TNF-α）和内皮素-1（ET-1）的浓度，作为促炎细胞因子，细胞凋亡和局部微循环的指标。此外，通过实时荧光定量PCR检测TNF-α和ET-1的mRNA含量，以验证其对基因表达的影响。结果：腋臭弧菌水提取物显着改善了乙醇给药引起的胃粘膜损伤（P <0.01）。乙醇给药后促炎细胞因子，TNF-α和ET-1升高，而V. axillare的水提取物则明显降低。腋臭水提取物也能抑制TNF-α和ET-1 mRNA的表达。结论：现有证据表明，腋臭水提取物可有效抵抗乙醇诱导的胃粘膜损伤，显着抑制促炎细胞因子的产生以及TNF-α和ET-1 mRNA的表达，这可能是因为对抑制细胞凋亡和改善局部微循环有用。

Study on the antiulcer effects of Veronicastrum axillare on gastric ulcer in rats induced by ethanol based on tumor necrosis factor-α (TNF-α) and endothelin-1 (ET-1)

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