High glucose induces myocardial cell injury through increasing reactive oxygen species production

Yu-Jun Wang; Xiao-Yu Lyu; Li Yu

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High glucose induces myocardial cell injury through increasing reactive oxygen species production

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Objective: To study the injury effect and molecular mechanism of high glucose on myocardial cells. Methods: Myocardial cells H9c2 were cultured and divided into the control group treated with DMEM containing 5.5 mmol/L glucose, the high glucose group treated with DMEM containing 35 mmol/L glucose, and the N-acetylcysteine (NAC) group pre-treated with 1000 μmol/L NAC and treated with DMEM containing 1000 μmol/L NAC and 35 mmol/L glucose. The production of ROS and the expression of mitochondria pathway apoptosis molecules in cells as well as the contents of collagen and collagen metabolism molecules were measured. Results: After 8 h, 16 hand 24 h of treatment, ROS RFU as well as Bax, CytC,Caspase-3 and Caspase-9 protein expression in cells and Col-I,Col-Ⅲ,PINP and PIIINP protein levels in culture medium of high glucose group were higher than those of control group,Bcl-2 protein expression were lower than those of control group, but CTX-I protein levels in culture medium were not significantly different from those of control group; after 24 h of treatment, Bax, CytC, Caspase-3 and Caspase-9 protein expression in cells as well as Col-I,Col-III, PINP and PIIINP protein levels in culture medium of NAC group were lower than those of high glucose group whereas Bcl-2 protein expression was higher than that of high glucose group. Conclusions: High glucose can induce myocardial cell apoptosis, increase collagen synthesis and accelerate interstitial fibrosis by increasing the production of reactive oxygen species.

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《亚太热带医药杂志（英文版）》 |2018年第001期|63-67|共5页
作者
Yu-Jun Wang; Xiao-Yu Lyu; Li Yu;
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Department of Critical Care Medicine, the Central Hospilal of Wuhan, Tongji Medical College, Huazhong Universily of Science and Technology, Wuhan Cuy, Hubei Province 430014, China;

Department of Endocrinology, the Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan City, Hubei Province, 430014, China;

Department of Critical Care Medicine, the Central Hospilal of Wuhan, Tongji Medical College, Huazhong Universily of Science and Technology, Wuhan Cuy, Hubei Province 430014, China;

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High glucose induces myocardial cell injury through increasing reactive oxygen species production

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