HSP70 inhibitor combined with cisplatin suppresses the cervical cancer proliferation in vitro and transplanted tumor growth: An experimental study

Jian Liu; Jing Liu; Su-Yang Guo; Hong-Li Liu; Sheng-Ze Li

首页> 中文期刊> 《亚太热带医药杂志（英文版）》 >HSP70 inhibitor combined with cisplatin suppresses the cervical cancer proliferation in vitro and transplanted tumor growth: An experimental study

HSP70 inhibitor combined with cisplatin suppresses the cervical cancer proliferation in vitro and transplanted tumor growth: An experimental study

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Objective:To study the regulating effect of HSP70 inhibitor (PES) combined with cisplatin on cervical cancer proliferationin vitro and transplanted tumor growth.Methods:Cervical cancer Hela cell lines were cultured and divided into control group, cisplatin group, PES group and cisplatin+PES group that were treated with serum-free DMEM, cisplatin with final concentration of 10 μmol/L, PES 20 μmol/L and cisplatin 10 μmol/L combined with PES with 20 μmol/L, respectively; animal models with cervical cancer xenografts were established and divided into control group, cisplatin group, PES group and cisplatin+PES group who received intra-tumor injection of normal saline, 10 μmol/L cisplatin, 20 μmol/L PES as well as 10 μmol/L cisplatin+20 μmol/L PES, respectively. Cell proliferation activity, transplanted tumor volume and mitochondria apoptosis molecule expression were detected.Results: Cell viability value and Bcl-2 mRNA expression in cells of cisplatin group, PES group and cisplatin+PES group were significantly lower than those of control group while Bax, Caspase-3 and Caspase-9 mRNA expression in cells were significantly higher than those of control group; transplanted tumor volume and the Bcl-2 mRNA expression in transplanted tumor tissue of cisplatin group, PES group and cisplatin+PES group were significantly lower than those of control group while Bax, Caspase-3 and Caspase-9 mRNA expression in transplanted tumor tissue were significantly higher than those of control group.Conclusions:HSP70 inhibitor combined with cislatin can inhibit cervical cancer cell proliferation in vitro and transplanted tumor growth through mitochondrial apoptosis pathway.

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《亚太热带医药杂志（英文版）》 |2017年第2期|177-181|共5页
作者
Jian Liu; Jing Liu; Su-Yang Guo; Hong-Li Liu; Sheng-Ze Li;
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Gynecological Oncology, the First Affiliated Hospital of Bengbu Medical College, Bengbu City 233004, Anhui Province, China;

Gynecological Oncology, the First Affiliated Hospital of Bengbu Medical College, Bengbu City 233004, Anhui Province, China;

Gynecological Oncology, the First Affiliated Hospital of Bengbu Medical College, Bengbu City 233004, Anhui Province, China;

Gynecological Oncology, the First Affiliated Hospital of Bengbu Medical College, Bengbu City 233004, Anhui Province, China;

Gynecological Oncology, the First Affiliated Hospital of Bengbu Medical College, Bengbu City 233004, Anhui Province, China;

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HSP70 inhibitor combined with cisplatin suppresses the cervical cancer proliferation in vitro and transplanted tumor growth: An experimental study

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