Nested multiplex PCR for identification and detection of human Plasmodium species including Plasmodium knowlesi

Maria Miguel-Oteo; Adela I Jiram; Thuy H Ta-Tang; Marta Lanza; Shamilah Hisam; José M Rubio

首页> 中文期刊> 《亚太热带医药杂志（英文版）》 >Nested multiplex PCR for identification and detection of human Plasmodium species including Plasmodium knowlesi

Nested multiplex PCR for identification and detection of human Plasmodium species including Plasmodium knowlesi

开具论文收录证明 >>

期刊封面封底目录下载 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Objective: To develop a new technique for diagnosis of Plasmodium knowlesi and at the same time to be able to discriminate among the diverse species of Plasmodium causing human malaria. Methods: In this study the nested multiplex malaria PCR was redesigned, targeting the 18S rRNA gene, to identify the fifth human Plasmodium species, Plasmodium knowlesi, together with the other human Plasmodium (Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale and Plasmodium malariae) by amplified fragment size using only two amplification processes and including an internal reaction control to avoid false negatives. Results: The technique was validated with 91 clinical samples obtained from patients with malaria compatible symptoms. The technique showed high sensitivity (100%) and specificity (96%) when it was compared to the reference method employed for malaria diagnosis in the Instituto de Salud Carlos Ⅲ and a published real-time PCR malaria assay. Conclusions: The technique designed is an economical, sensitive and specific alternative to current diagnosis methods. Furthermore, the method might be tested in knowlesi-malaria endemic areas with a higher number of samples to confirm the quality of the method.

著录项

来源
《亚太热带医药杂志（英文版）》 |2017年第3期|280-284|共5页
作者
Maria Miguel-Oteo; Adela I Jiram; Thuy H Ta-Tang; Marta Lanza; Shamilah Hisam; José M Rubio;
展开▼
作者单位

Malaria & Emerging Parasitic Diseases Laboratory, Parasitology Department, National Microbiology Centre. Instituto de Salud Carlos Ⅲ, Cra. Majadahonda Pozuelo Km. 2, Majadahonda, 28220 Madrid, Spain;

Parasitology Unit, Institut Penyelidikan Perubatan, Institute for Medical Research, 50588 Jalan Pahang, Kuala Lumpur, Malaysia;

Malaria & Emerging Parasitic Diseases Laboratory, Parasitology Department, National Microbiology Centre. Instituto de Salud Carlos Ⅲ, Cra. Majadahonda Pozuelo Km. 2, Majadahonda, 28220 Madrid, Spain;

Malaria & Emerging Parasitic Diseases Laboratory, Parasitology Department, National Microbiology Centre. Instituto de Salud Carlos Ⅲ, Cra. Majadahonda Pozuelo Km. 2, Majadahonda, 28220 Madrid, Spain;

Parasitology Unit, Institut Penyelidikan Perubatan, Institute for Medical Research, 50588 Jalan Pahang, Kuala Lumpur, Malaysia;

Malaria & Emerging Parasitic Diseases Laboratory, Parasitology Department, National Microbiology Centre. Instituto de Salud Carlos Ⅲ, Cra. Majadahonda Pozuelo Km. 2, Majadahonda, 28220 Madrid, Spain;

展开▼
原文格式 PDF
正文语种 eng
中图分类
关键词

Nested multiplex PCR for identification and detection of human Plasmodium species including Plasmodium knowlesi

摘要

著录项

相关主题

期刊订阅