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首页> 中文期刊> 《亚太热带医药杂志（英文版）》 >Effect of recombinant human endostatin onradiotherapy for esophagus cancer

Effect of recombinant human endostatin onradiotherapy for esophagus cancer

         
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摘要

Objective: To investigate the effect of radiotherapy plus recombinant human endostatin (RH-endostatin) on esophageal cancer and its mechanism. Methods: A total of 50 nudemice were equally randomized into control group, radiotherapy group, and combined therapy group Ⅰ, Ⅱ,andⅢ after inoculating with Eca109 cell suspension (1×10 7 cells/mL). On the day of grouping, control group and radiotherapy group were injected normal saline, while radiotherapy group and 3 combined therapy groups received radiotherapy; besides, combined therapy group Ⅰ, Ⅱ,andⅢ was injected RH-endostatin of 2.5, 5, 10 mg/kg respectively. After 3-week therapy, the tumors of each group were collected and microvessel density and VEGF expression in tumors were determined. In vitro, Eca109 cells were divided into control group, radiotherapy group, and combined therapy group. Forty-eight hours after treatment, cell cycle distribution and apoptosis rate were detected, and the activity of VEGF signal paths was semiquantitatively analyzed. Results: Since the 6th day of treatment, the relative tumor proliferation rate of combined therapy group Ⅱ was lower than radiotherapy group (P<0.05) and ≤40% since the 15th day. Average microvessel density and EGFR expression in combined therapy group Ⅱ were lower thanradiotherapy group (P<0.05). In vitro, the cell percentage in S and G 2/M phase of combined therapy group cells was lower than that in radiotherapy group cells, while the apoptosis rate and the expression of VEGF, AKT, p-AKT, ERK1/2 and p-ERK1/2 in combined group were higher than that in radiotherapy group (P<0.05). Conclusions: RH-endostatin promotes the efficacy of radiotherapy on esophageal cancer, which may be partly realized by inhibiting the activity of VEGF related signal paths.

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  • 来源
    《亚太热带医药杂志（英文版）》 |2016年第1期|88-92|共5页
  • 作者

    Gao-Feng Liu; Hui Chang; Bao-Tian Li; Yong Zhang; Dan-Dan Li; Yan Liu; Yang Yang;

  • 作者单位

    Department of Thoracic Surgery, No.153 Hospital of Liberation Army, Zhengzhou, China;

    Department of Thoracic Surgery, No.153 Hospital of Liberation Army, Zhengzhou, China;

    Department of Thoracic Surgery, No.153 Hospital of Liberation Army, Zhengzhou, China;

    Department of Thoracic Surgery, No.153 Hospital of Liberation Army, Zhengzhou, China;

    Department of Thoracic Surgery, No.153 Hospital of Liberation Army, Zhengzhou, China;

    Department of Thoracic Surgery, No.153 Hospital of Liberation Army, Zhengzhou, China;

    Departmen of Cardiothoracic Surgery, Xiangya Hospital Central-South University. Changsha, China;

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