Evolutionary relationship of 5'-untranslated regions among Thai dengue-3 viruses, Bangkok isolates, during 24 year-evolution

Watcharee Attatippaholkun; Panyupa Pankhong; Ananda Nisalak; Siripen Kalayanarooj

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Evolutionary relationship of 5'-untranslated regions among Thai dengue-3 viruses, Bangkok isolates, during 24 year-evolution

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Objective:To study evolutionary relationship of the 5'untranslated regions(5'UTRs) in low passage dengue3 viruses(DEN3) isolated from hospitalized children with different clinical manifestations in Bangkok during 24 year-evolution(1977-2000) comparing to the DEN3prototype(H87).Methods:The 5'UTRs of these Thai DEN3 and the H87 prototype were amplified by RT-PCR and sequenced.Their multiple sequence alignments were done by Codon Code Aligner v 4.0.4 software and their RNA secondary structures were predicted by MFOLD software.Replication of five Thai DEN3 candidates comparing to the 1187 prototype were done in human(HepG2) and the mosquito(C6/36) cell lines.Results:Among these Thai DEN3,the completely identical sequences of their first 89 nucleotides,their high-order secondary structure of 5'UTRs and three SNPs including the predominant C90 T,and two minor SNPs including A109 G and A112 G were found.The C90 T of Thai DEN3.Bangkok isolates was shown predominantly before 1977.Five Thai DEN3 candidates with the predominant C90 T were shown to replicate in human(HepG2) and the mosquito(C6/36) cell lines better than the H87 prototype.However,their highly conserved sequences as well as SNPs of the 5'UTR did not appear to correlate with their disease severity in human.Conclusions:Our findings highlighted evolutionary relationship of the completely identical 89 nucleotide sequence,the high-order secondary structure and the predominant C90 T of the 5'UTR of these Thai DEN3 during 24 year-evolution further suggesting to be their genetic markers and magic targets for future research on antiviral therapy as well as vaccine approaches of Thai DEN3.

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《亚太热带医药杂志（英文版）》 |2015年第3期|176-184|共9页
作者
Watcharee Attatippaholkun; Panyupa Pankhong; Ananda Nisalak; Siripen Kalayanarooj;
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Department of Clinical Chemistry, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand;

Department of Clinical Chemistry, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand;

Department of Virology, US Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok 10400, Thailand;

Queen Sirikit National Institute of Child Health, Bangkok 10400, Thailand;

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Evolutionary relationship of 5'-untranslated regions among Thai dengue-3 viruses, Bangkok isolates, during 24 year-evolution

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