Effects of high glucose on expression of OPG and RANKL in rat aortic vascular smooth muscle cells

Hong-Juan Chang; Tian-Fa Li; Jun-Li Guo; You-Ling Lan; Yue-Qiong Kong; Xin Meng; Xian-Ji Ma

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Effects of high glucose on expression of OPG and RANKL in rat aortic vascular smooth muscle cells

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Objective:To explore effect of high glucose on expression of osteoprotegerin(OPG) and receptor activator of NF- κB ligand(RANKL) in rat aortic vascular smooth muscle cells.Methods:SD rats were intraperitoneally injected with streptozotocin,OPG and RANKL expression in rat thoracic aortas were detected by immunohistochemical staining.In cultured vascular smooth muscle cells(VSMCs)(A7r5),qRT-PCR and Western blot analysis were used to examine the mRNA and protein levels of OPG and RANKL.Results:Our results demonstrated that OPG expression was increased in hyperglycemic rat aortic VSMCs.while RANKL expression was decreased.Besides,in vitro experiments high glucose induced OPG expression,but depressed RANKL expression by dose- and time-dependent manner in cultured A7r5.Conclusions:Our findings suggested that high glucose could promote the expression of OPG,and inhibit the expression of RANKL in VSMCs,which may be partly be the molecular mechanism of diabetic vascular calcification.

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《亚太热带医药杂志（英文版）》 |2015年第3期|209-213|共5页
作者
Hong-Juan Chang; Tian-Fa Li; Jun-Li Guo; You-Ling Lan; Yue-Qiong Kong; Xin Meng; Xian-Ji Ma;
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Department of Neurology, the Second People's Hospital of Xinxiang, Xinxiang 453002, PR China;

Department of Cardiovascular Institute, Affiliated Hospital of Hainan Medical College, Haiku 571199, PR China;

Department of Cardiovascular Institute, Affiliated Hospital of Hainan Medical College, Haiku 571199, PR China;

Department of Cardiovascular Institute, Affiliated Hospital of Hainan Medical College, Haiku 571199, PR China;

Department of Cardiovascular Institute, Affiliated Hospital of Hainan Medical College, Haiku 571199, PR China;

Department of Neurology, the Second People's Hospital of Xinxiang, Xinxiang 453002, PR China;

Department of Cardiovascular Institute, Affiliated Hospital of Hainan Medical College, Haiku 571199, PR China;

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Effects of high glucose on expression of OPG and RANKL in rat aortic vascular smooth muscle cells

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