Isolation, fractionation and evaluation of the antiplasmodial properties of Phyllanthus niruri resident in its chloroform fraction

Obidike Ifeoma; Okhale Samuel; Aboh Mercy Itohan; Salawu Oluwakanyinsola Adeola

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Isolation, fractionation and evaluation of the antiplasmodial properties of Phyllanthus niruri resident in its chloroform fraction

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Objective:To investigate the antiplasmodial activity ofPhyllanthus niruri(P. niruri) methanol extract(ME) and its fractions in mice.Methods:P. niruri methanol extract and its chloroform, ethanol and aqueous portions were tested against chloroquine-sensitivePlasmodium berghei bergheiin early, established and repository models of infection usingKnight andPeter's4-day suppressive model,Ryley andPeters curative model andPeters prophylactic model respectively. Results:Chemosuppression of parasitaemia(37.65%-50.53%) was elicited by100-400 mg/kg(b.w.) ofME.At doses of100 mg/kg b.w., the chloroform fraction(F1) significantly(P<0.01) suppressed parasitaemia by85.29%, while ethanol and aqueous fractions(F2 andF3, respectively) elicited 67.06% and51.18% chemosuppression.The most active fraction,F1 was selected for further antiplasmodial screening.Inestablished infection,ME reduced parasitaemia(15.81% -62.96%) whileF1 significantly(P<0.01) reduced parasitaemia(44.36%-90.48%), with effects comparable to that of chloroquine(96.48%).The prophylactic antiplasmodial activity ofME(92.50% suppression) was also significant(P<0.01) and was more effective than pyrimethamine(85.00%).Additionally, cell membrane integrity of non-parasitizederythrocytes incubated with125-500 mg/mLF1 was maintained.Conclusions:These findings indicate the antiplasmodial efficacy ofP. niruri methanol extract, and the localization of this effect in its chloroform fraction.

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《亚太热带医药杂志（英文版）》 |2013年第3期|169-175|共7页
作者
Obidike Ifeoma; Okhale Samuel; Aboh Mercy Itohan; Salawu Oluwakanyinsola Adeola;
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Department of Pharmacology and Toxicology, National Institute for Pharmaceutical Research and Development, P.M.B. 21, Abuja, Nigeria;

Department of Medicinal Plant Research and Traditional Medicine, National Institute for Pharmaceutical Research and Development, P.M.B. 21, Abuja, Nigeria;

Department of Microbiology and Biotechnology, National Institute for Pharmaceutical Research and Development, P.M.B. 21, Abuja, Nigeria;

Department of Pharmacology and Toxicology, National Institute for Pharmaceutical Research and Development, P.M.B. 21, Abuja, Nigeria;

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Isolation, fractionation and evaluation of the antiplasmodial properties of Phyllanthus niruri resident in its chloroform fraction

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