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首页> 外文期刊>亚太热带医药杂志（英文版） >Prospective study of SEVA TB peroxidase assay for cocktail antigen and antibody in the diagnosis of Tuberculosis in suspected patients attending a tertiary care hospital located in rural area
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Prospective study of SEVA TB peroxidase assay for cocktail antigen and antibody in the diagnosis of Tuberculosis in suspected patients attending a tertiary care hospital located in rural area

机译：SEVA TB过氧化物酶测定鸡尾酒蛋白和抗体的前瞻性研究在农村地区三级医院就诊的疑似患者中诊断肺结核

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Objective:To evaluate inhouse developed SEVA TB peroxidase enzyme immunoassay using cocktail of mycobacterial excretory-secretory antigens (ES-31, ES-43&EST-6) for antibody detection and their affinity purified antibodies for antigen detection in tuberculosis suspected patients. Methods:Inhouse developed SEVA TB peroxidase enzyme immunoassay was evaluated prospectively in 73 suspected pulmonary and 46 extra-pulmonary tuberculosis patients during November 2008~March 2009 in a tertiary hospital located in rural area. Results: Assay on prospective analysis showed 100% correlation of pulmonary tuberculosis (PTB) and extra-pulmonary tuberculosis (EPTB) acid fast bacilli positivity and antitubercular treatment in 11 cases. Thirty nine PTB and 12 EPTB cases showed negative for ELISA test and were also not given antitubercular therapy. However 30 PTB and 27 EPTB cases showing ELISA positivity were neither acid fast bacilli positive nor antitubercular therapy treated. These cases may possibly have dormant infection and need further diagnosis. In EPTB cases ELISA was observed to be more useful than AFB smear test. Conclusions:This inhouse developed user-friendly peroxidase ELISA can be used as an adjunct test of smear microscopy or culture techniques for routine screening of patients suspected of PTB or EPTB.
机译：目的：使用结核分枝杆菌分泌分泌抗原（ES-31，ES-43＆EST-6）的混合物检测内部开发的SEVA TB过氧化物酶酶免疫法，以检测疑似结核病患者的抗体，并使用亲和纯化的抗体进行抗原检测。方法：对2008年11月至2009年3月在农村地区一家三级医院内对73例疑似肺病和46例肺外结核患者进行的内部开发的SEVA TB过氧化物酶免疫测定进行了前瞻性评估。结果：前瞻性分析结果显示11例肺结核（PTB）和肺外结核（EPTB）耐酸快速细菌阳性率与100％的相关性。 39例PTB和12例EPTB的ELISA检测呈阴性，也未给予抗结核治疗。然而，显示ELISA阳性的30例PTB和27例EPTB既不耐酸杆菌阳性也不接受抗结核治疗。这些病例可能有潜伏感染，需要进一步诊断。在EPTB病例中，观察到ELISA比AFB涂片检查更有用。结论：该内部开发的用户友好型过氧化物酶ELISA可以用作涂片显微镜或培养技术的辅助测试，用于常规筛查疑似PTB或EPTB的患者。

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