首页> 中文期刊> 《亚太热带医药杂志(英文版)》 >Optimized combinations of statins and azoles against Acanthamoeba trophozoites and cysts in vitro

Optimized combinations of statins and azoles against Acanthamoeba trophozoites and cysts in vitro

         

摘要

Objective: To evaluate the combination of several statins (atorvastatin, fluvastatin and simvastatin) and azoles (voriconazole, posaconazole and itraconazole) against Acanthamoeba spp. Methods: The efficiency of the different drug combinations against the trophozoite stage of different Acanthamoeba strains were evaluated by Alamar Blue assay. Effect on the cyst stage was observed by inverted microscope. Cytotoxicity of combinations of azoles and statins was evaluated by measuring the release of lactate dehydrogenase from a murine macrophage cell line. Results: Combinations of any of the tested statins and voriconazole or posaconazole were more efficient in inhibiting Acanthamoeba compared to statins or azoles individually. The drug combinations at the combined inhibitory concentrations 50% showed lower toxicity compared to that of the compounds alone. Conclusions: The combinations of statins together with voriconazole and posaconazole are more efficient than these drugs alone, and these combinations have lower cytotoxicity in mammalian cell lines.

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  • 来源
    《亚太热带医药杂志(英文版)》 |2019年第6期|283-287|共5页
  • 作者单位

    University Institute of Tropical Diseases and Public Health of the Canary Islands;

    University of La Laguna;

    Canary Islands;

    Spain;

    Laboratoire Matériaux-Molécules et Applications;

    IPEST;

    LA Marsa;

    University of Carthage;

    Tunisia;

    University Institute of Tropical Diseases and Public Health of the Canary Islands;

    University of La Laguna;

    Canary Islands;

    Spain;

    University Institute of Tropical Diseases and Public Health of the Canary Islands;

    University of La Laguna;

    Canary Islands;

    Spain;

    University Institute of Tropical Diseases and Public Health of the Canary Islands;

    University of La Laguna;

    Canary Islands;

    Spain;

    University Institute of Tropical Diseases and Public Health of the Canary Islands;

    University of La Laguna;

    Canary Islands;

    Spain;

    University Institute of Tropical Diseases and Public Health of the Canary Islands;

    University of La Laguna;

    Canary Islands;

    Spain;

    Centre for Integrative Physiology;

    Biomedical Sciences;

    Edinburgh Medical School;

    University of Edinburgh;

    Scotland;

    UK;

    University Institute of Tropical Diseases and Public Health of the Canary Islands;

    University of La Laguna;

    Canary Islands;

    Spain;

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  • 正文语种 eng
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