碳纳米材料由于其具有优异的性能,得以广泛生产和使用,其不可避免会进入水环境中,对水生生态系统造成潜在影响.多壁碳纳米管(P-MWCNTs)和羟基化多壁碳纳米管(MWCNTs-OH)作为纳米材料的典型代表,应用非常广泛,其潜在的环境效应受到人们越来越多的关注.为此,本文以蛋白核小球藻(Chlorella pyrenoidosa)作为受试生物,通过暴露实验,研究了P-MWCNTs和MWCNTs-OH对蛋白核小球藻的生物学效应.研究结果表明:1)当P-MWCNTs浓度≤10 mg·L-1、MWCNTs-OH≤20 mg·L-1浓度时对蛋白核小球藻生长未造成影响;2)暴露96 h后,当P-MWCNTs≤10 mg·L-1、MWCNTs-OH浓度≤20 mg·L-1时,蛋白核小球藻细胞可溶性蛋白质含量增加,当P-MWCNTs浓度≥20 mg·L-1、MWCNTs-OH浓度≥40 mg·L-1时,2种类型MWCNTs均对蛋白核小球藻造成毒性效应;3)随着2种类型MWCNTs浓度的增加,蛋白核小球藻细胞总抗氧化能力(T-AOC)值减少,蛋白核小球藻细胞丙二醛(MDA)含量显著增加,细胞的健康程度逐渐恶化,细胞结构受到严重损伤;4)MWCNTs-OH比P-MWCNTs具有更好的生物相容性.%Due to the excellent characteristics of carbon nanomaterials, they are widely produced and applied in many fields. Thus, they will inevitably enter into water environment and cause potential impact on the aquatic ecosystems. Multi-walled carbon nanotubes (P-MWCNTs) and hydroxylated multi-walled carbon nanotubes (MWCNTs-OH), as two typical materials of nanomaterials, are widely applied and their potential environmental effects are attracting more and more attention. In the present study, the toxicology effects of P-MWCNTs and MWCNTs-OH on Chlorella pyrenoidosa were investigated via exposure experiment. The results showed that P-MWCNTs had littleinfluence on the growth of Chlorella pyrenoidosa when its concentration was 10 mg·L-1 or less and MWCNTs-OH had little influence on the growth of Chlorella pyrenoidosa when its concentration was 20 mg·L-1 or less. Secondly, after96 h, the produce of soluble protein was promoted when P-MWCNTs concentration was 10 mg·L-1 or less and MWCNTs-OH concentration was 20 mg·L-1 or less. Moreover, when P-MWCNTs concentration reached 20 mg·L-1 and MWCNTs-OH concentration reached 40 mg·L-1 respectively, two types of carbon nanotubes exhibited significant toxic effects on Chlorella pyrenoidosa. Thirdly, with the increasing concentration of two types of carbon nanotubes respectively, the total antioxidant capacity (T-AOC) values of Chlorella pyrenoidosa cells decreased, while malonlydialdehyde (MDA) content in Chlorella pyrenoidosa increased notably and the health state of cells was gradually deteriorated, which indicated that the cells structure was destroyed severely. Moreover, MWCNTs-OH has better biocompatibility than P-MWCNTs.
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