Inhibition of Mahkota Dewa(Phaleria macrocarpa) bioactive fraction on proliferation of human retinoblastoma tumor cells Y-79 through suppression of mRNA level of cyclin E

Nugroho Trilaksana; Ignatius Riwanto; Raymond Rubianto Tjandrawinata; Reki Winarto

首页> 中文期刊> 《亚太热带生物医学杂志：英文版》 >Inhibition of Mahkota Dewa(Phaleria macrocarpa) bioactive fraction on proliferation of human retinoblastoma tumor cells Y-79 through suppression of mRNA level of cyclin E

Inhibition of Mahkota Dewa(Phaleria macrocarpa) bioactive fraction on proliferation of human retinoblastoma tumor cells Y-79 through suppression of mRNA level of cyclin E

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Objective: To prove the molecular mechanisms of Mahkota Dewa(Phaleria macrocarpa) in suppressing proliferation of human retinoblastoma cells through suppression of cell cycle’s gene-regulators expression.Methods: In this study, the molecular mechanism of anti-tumor effect of fractioned extract of Phaleria macrocarpa(DLBS1425) in human retinoblastoma cells Y-79 was investigated by measuring the tumor cells viability, the assessment of population profiles of tumor cells in the cell cycle, and the mRNA concentration of p16, p21, p53, cyclin D,cyclin E, and E2 F.Results: DLBS1425 showed an inhibition effects towards proliferation of Y-79 cell line.Inhibition of proliferation was shown by suppression of cell cycle progression.DLBS1425 downregulated cyclin E, a G1 phase regulator gene of cell cycle, in dosedependent manner without affecting p53–p21 pathway.In the other word, DLBS1425 inhibits cell proliferation through suppression of cyclin E independently towards conventional proliferation pathway.Conclusions: Our results suggest that DLBS1425 is a potential anticancer agent which targets genes involved in cell proliferation in human retinoblastoma cells which make it pharmacologically ideal for the prevention and/or treatment of retinoblastoma cancer.

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《亚太热带生物医学杂志：英文版》 |2017年第004期|P.280-287|共8页
作者
Nugroho Trilaksana; Ignatius Riwanto; Raymond Rubianto Tjandrawinata; Reki Winarto;
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作者单位

Department of Ophthalmology,Faculty of Medicine,Diponegoro University;

Department of Surgery,Faculty of Medicine,Diponegoro University;

Division of Molecular Pharmacology and Phytochemistry,Dexa Laboratories of Biomolecular Sciences,Dexa Medica Group;

Department of Ophthalmology,Faculty of Medicine,Diponegoro University;

Department of Surgery,Faculty of Medicine,Diponegoro University;

Division of Molecular Pharmacology and Phytochemistry,Dexa Laboratories of Biomolecular Sciences,Dexa Medica Group;

Department of Ophthalmology,Faculty of Medicine,Diponegoro University;

Department of Surgery,Faculty of Medicine,Diponegoro University;

Division of Molecular Pharmacology and Phytochemistry,Dexa Laboratories of Biomolecular Sciences,Dexa Medica Group;

Department of Ophthalmology,Faculty of Medicine,Diponegoro University;

Department of Surgery,Faculty of Medicine,Diponegoro University;

Division of Molecular Pharmacology and Phytochemistry,Dexa Laboratories of Biomolecular Sciences,Dexa Medica Group;

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Inhibition of Mahkota Dewa(Phaleria macrocarpa) bioactive fraction on proliferation of human retinoblastoma tumor cells Y-79 through suppression of mRNA level of cyclin E

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