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首页> 外文期刊>亚太热带生物医学杂志：英文版 >Effect of extracts from Stachys sieboldii Miq. on cellular reactive oxygen species and glutathione production and genomic DNA oxidation
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Effect of extracts from Stachys sieboldii Miq. on cellular reactive oxygen species and glutathione production and genomic DNA oxidation

机译：水飞蓟提取物的作用。活性氧和谷胱甘肽的产生及基因组DNA的氧化

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摘要

Objective: To evaluate the antioxidant activity of extracts and fractions from Stachys sieboldii Miq., and to examine its effect on the cellular reactive oxygen species(ROS) and glutathione(GSH) production and genomic DNA oxidation in HT-1080 cells. Methods: The ROS generation induced by H2 O2 was measured by the dichlorofluorescein-diacetate assay. GSH levels were measured using a fluorescent method with mBBr. Genomic DNA oxidative damage was measured with levels of oxidative DNA induced by the reaction of ferritin with H2 O2. Results: The n-hexane, 85% aqueous methanol and n-butanol fractions(0.05 mg/mL concentrations) inhibited H2 O2-induced ROS generation by 63%, 35% and 45%, respectively. GSH levels were significantly increased in both acetone+methylene chloride and methanol extracts(P<0.05). Supplementation of cells with n-hexane significantly increased GSH levels at concentrations of 0.05 mg/mL(P<0.05). Both the acetone+methylene chloride and methanol extracts, as well as all fractions significantly inhibited oxidative DNA damage(P<0.05). Conclusions: These results indicate that cellular oxidation was inhibited by the n-hexane fraction and this fraction may contain valuable active compounds.
机译：目的：评价水苏木提取物和级分的抗氧化活性，并研究其对HT-1080细胞中细胞活性氧（ROS）和谷胱甘肽（GSH）生成及基因组DNA氧化的影响。方法：用二氯荧光素-二乙酸盐法测定过氧化氢诱导的ROS生成。 GSH水平是使用mBBr荧光法测量的。用铁蛋白与H 2 O 2反应诱导的氧化DNA水平来测量基因组DNA的氧化损伤。结果：正己烷，85％的甲醇水溶液和正丁醇馏分（0.05 mg / mL浓度）分别抑制H2 O2诱导的ROS生成，分别抑制63％，35％和45％。丙酮+二氯甲烷和甲醇提取物中的谷胱甘肽水平显着增加（P <0.05）。在浓度为0.05 mg / mL的情况下，向细胞添加正己烷可显着提高GSH水平（P <0.05）。丙酮+二氯甲烷和甲醇提取物，以及所有馏分均显着抑制DNA的氧化损伤（P <0.05）。结论：这些结果表明，正己烷馏分可抑制细胞氧化，并且该馏分可能包含有价值的活性化合物。

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