• 主题
高级搜索  >
历史搜索 清空历史
首页> 中文期刊> 《生物医学与环境科学：英文版》 >Effects of Copper-phenanthroline on Pentachlorophenol-induced Adaptation and Cell Death of Escherichia coli

Effects of Copper-phenanthroline on Pentachlorophenol-induced Adaptation and Cell Death of Escherichia coli

         
页面导航
  • 摘要
  • 著录项
  • 相关主题

摘要

Objective To evaluate the effects of copper-phenanthroline(CuOP) on pentachlorophenol(PCP)-induced adaptation and cell death of Escherichia coli. Methods Bacterial growth and adaptation to PCP were monitored spectrophotometrically at 600 nm. Inactivation of bacterial cells was determined from colony count on agar dishes. Cellular ATP content and accumulation of PCP were assessed by chemiluminescence and HPLC analysis respectively. The formation of PCP-Cu-OP complex was shown by UV-visible spectra. Results Escherichia coli(E. coli) could adapt to PCP,a wood preservative and insecticide used in agriculture. The adaptation of E. coli to PCP prevented its death to the synergistic cytotoxicity of CuOP plus PCP and declined cellular accumulation and uncoupling of oxidative phosphorylation of PCP. Furthermore,CuOP and PCP neither produced reactive oxygen species(ROS) nor had a synergistic effect on uncoupling of oxidative phosphorylation in E. coli. The synergistic cytotoxicity of CuOP and PCP in E. coli might be due to the formation of lipophilic PCP-Cu-OP complex. Conclusion Our data suggested that adaptation of E. coli to PCP decreased the synergistic effects of CuOP and PCP on prokaryotic cell death due to the formation of lipophilic PCP-Cu-OP complex,but it had no effect on the uncoupling of oxidative phosphorylation and production of reactive oxygen species in E. coli.

著录项

获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号