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首页> 外文期刊>生物医学与环境科学（英文版） >Proteome Analysis of Neisseria meningitidis Serogroup Strains C Associated with Outbreaks in China
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Proteome Analysis of Neisseria meningitidis Serogroup Strains C Associated with Outbreaks in China

机译：脑膜炎奈瑟氏球菌血清群C与中国暴发相关的蛋白质组学分析

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摘要

Objective During 2003-2005, an outbreak of meningitis due to Neisseria meningitidis serogroup C occurred in China. With the aim to find strain clues result in the final epidemics, the ancestral strain 053442, a clinical isolate, and a carrier strain 053426 with different gene type were analyzed. Methods Clinical strain 053442 and carrier strain 053426 were cultured on GC agar plates under the same condition. Two-dimensional electrophoresis was performed using the pH 3-10 nonlinear IPG strips of 24 cm length, and all the protein spots were identified by matrix-assisted laser desorption/ionization time of flight spectrometry. Results 502 and 380 protein spots were identified in 053426 and 053442 respectively, relating to 266 and 202 different genes covering a wide range of cellular functions. The express volume and number of proteins involved in energy metabolism, protein synthesis and amino acid biosynthesis in 053426 were higher than in 053442. Virulence factor Opa, Opc and a series of proteins involved in pilus assembly and retraction were identified in 053442, which appear to be of primary importance in colonization and invasion of human cells. Compared to 053442, virulence protein species were less in 053426, with lower express volumes too. No Opa and Opc were detected in 053426. Conclusion The different protein expression profiles of the clinical strain 053442 and carrier strain 053426 in the present study provide some clues of the different pathogenicity of the two strains, which may account for result in the final epidemics.

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  • 来源
    《生物医学与环境科学（英文版）》 |2010年第4期|251-258|共8页
  • 作者

    YUAN HU; LI-HUA HE; LI XU; YI-XIN GU; HAI JIANG; FENG-HUA GUO; ZHEN-WEI DAI; HONG-YU REN; MEI-JUAN LU; XIA CHEN; QING-HUA ZOU; ZHU-JUN SHAO; FAN-LIANG MENG; JIAN-ZHONG ZHANG; XIAO-MEI YAN; BO-QING LI; FEI ZHAO; DI XIAO; JUN REN; MING-HUAN ZHENG; CHUN-XIANG FAN;

  • 作者单位

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

    Anhui Provincial Center for Disease Control and Prevention,Hefei 230061,Anhui,China;

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

    Anhui Provincial Center for Disease Control and Prevention,Hefei 230061,Anhui,China;

    Anhui Provincial Center for Disease Control and Prevention,Hefei 230061,Anhui,China;

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

    Anhui Provincial Center for Disease Control and Prevention,Hefei 230061,Anhui,China;

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

    National Institute for Communicable Disease Control and Prevention,China CDC,PO Box 5,Changping,Beijing 102206,China;

  • 收录信息 中国科学引文数据库(CSCD);
  • 原文格式 PDF
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