简单重复系列区间(inter-simple sequence repeat,ISSR)是在简单重复序列(simple sequence repeat,SSR)基础上发展起来的一种新型分子标记技术.目前药用植物研究与开发面临资源枯竭、药效物质不明确及质量难以控制等难题,DNA分子标记技术,包括ISSR技术为上述难题提供了新的解决办法.比较了几种常用分子标记的优缺点,最后着重介绍了目前广泛应用于药用植物研究中的DNA分子标记ISSR的技术原理和特点,综述了ISSR分子标记技术在药用植物的遗传多样性和遗传结构、种质资源鉴定、中药品质鉴定,以及遗传图谱等方面的研究进展及应用前景,旨为药用植物的开发和利用提供参考.%Inter-simple sequence repeat(ISSR)has been known as a novel molecular maker technology based on simple sequence repeat(SSR). At present,the research and development of medicinal plants are facing the problems such as unclear drug substance, uncontrollable quality and exhausted resources. DNA molecular marker technology,especially ISSR,has provided a new solution for the above problems. Comparing the merits and demerits of several commonly used molecular markers,finally,we mainly introduced the principles and characteristics of DNA molecular marker ISSR applied in current research of the medicinal plants,and summarized the new advances on ISSR molecular technology and its application prospective in the genetic diversity and structure of medicinal plants,identification of germplasm resources,quality identification of traditional Chinese medicine,and genetic map,aiming at providing better reference for the development and utilization of medicinal plants.
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