旨在探讨生理性深层海水(PDSW)联合热疗对人胃癌SGC-7901细胞和组织中COX-2和Bcl-2表达的影响,明确其在人胃癌SGC-7901体外和体内的抗癌作用.通过在体外培养人胃癌SGC-7901细胞株,随机分为实验组、对照组和空白组,实验组加PDSW,对照组加生理盐水,空白组不做其他处理,分别在37℃、40℃和43℃热疗温度下培养.同时,将SGC-7901细胞注射至裸鼠皮下,建立移植瘤模型,随机分为5组,至肿瘤长至直径为1 cm时,分别进行H2O+RT、H2O+40℃、H2O+43℃、PDSW+40℃、PDSW+43℃处理,用实时荧光定量PCR检测细胞和组织中COX-2和Bcl-2基因表达情况,Western blot检测COX-2和Bcl-2蛋白的表达情况.结果显示,在SGC-7901细胞中和裸鼠皮下移植瘤的组织中,热疗均能降低COX-2、Bcl-2基因及蛋白的表达,同时,与生理盐水组相比,PDSW联合热疗处理下,细胞和组织中COX-2、Bcl-2表达量显著下降.PDSW联合热疗能抑制胃癌SGC-7901细胞中COX-2、Bcl-2基因和蛋白的表达,从而有效抑制胃癌细胞的增殖.%This work aims to explore the effect of PDSW combined with hyperthermia on COX-2 and Bcl-2 expressions in gastric cancer cells SGC-7901 and tissues,and to clarify its anti-cancer effects on gastric cancer cells SGC-7901 in vitro and in vivo. The SGC-7901 cells were cultured in vitro and then were randomly divided into experimental,control and blank groups ;the experimental group was added with PDSW, control group was given with normal saline,blank group was in no other treatment,and each group were cultured under 37℃,40℃ and 43℃respectively. Concurrently,the SGC-7901 cells were injected into the nude mice subcutaneous to establish the transplantation tumor model. The injected mice were randomly divided into 5 groups,and then given the treatment with H2O+RT,H2O +40℃,H2O +43℃,PDSW+40℃, and PDSW+43℃ respectively when the tumor cells grew to 1 cm diameter. qRT-PCR and Western blot were used to detect the mRNA of gene COX-2 and Bcl-2 and the protein expression of COX-2 and Bcl-2 in the cells and tissues. As results,in SGC-7901 cells and nude mice subcutaneous transplantation tumor tissues,the expressions of gene COX-2 and Bcl-2 mRNA and their protein expressions decreased under the treatment of hyperthermia. Compared with the treatment of normal saline group,the expressions of gene COX-2 and Bcl-2 and their protein expression decreased remarkably under the treatment of PDSW combined with hyperthermia. Conclusively,PDSW combined with hyperthermia may inhibit the expressions of gene COX-2 and Bcl-2 and their protein expressions in gastric cancer SGC-7901 cells and tissues,so as to effectively inhibit the proliferation of gastric cancer cells.
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