基于花生转录组及基因组数据，克隆获得一个花生的 NBS-LRR 类抗逆基因的全长序列，该基因与大豆抗病性蛋白基因（KHN40407.1）的相似性为79%，命名为 AhNDrp 基因。该基因不含内含子，cDNA 全长2832 bp，有5个典型的抗逆性蛋白保守结构域，其中 Domain Ⅴ是富含亮氨酸的重复序列。荧光定量 PCR 分析表明，AhNDrp 基因在花生根中特异性表达，且黄曲霉处理后该基因表达量显著上升，说明该基因可能参与抗病反应。%Based on peanut’s transcriptome and genome data,a full length sequence of NBS-LRR like-gene was cloned. This gene, which showed 79% similarity with disease resistance protein(KHN40407.1)of Glycine soja,was designated as AhNDrp gene. The length of cDNA of AhNDrp gene was 2 832 bp,and contained no intron. AhNDrp gene harbored 5 typical conserved domains with resistance-related protein,and Domain V was repeat sequence with rich leucine. Real-time quantitative PCR analysis showed that AhNDrp gene was expressed exclusively in root,and the expression was significantly increased after aflatoxin treatment,indicating that this gene was involved in the resistance response.
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