We collected the pituitary, ovary and other tissue samples of 5 Tibetan goats and 5 Jintang black goats at estrus stage, cloned cDNA of Apaf-1 and Apaf-2, analyzed their sequences, and investigated their mRNA expression of tissues by qPCR. The results showed that:the coding region sequence(CDS)of goat Apaf-1 gene was 3 750 bp long encoding 1 249 amino acids, and the CDS of Apaf-2 gene was 318 bp encoding 105 amino acids. The two genes showed the same sequences in both of goats, no mutation and no differential expression in 5 tissues. Our data suggested that gene Apaf-1 and Apaf-2 in animal’s evolution were conservative, and the correlation with the trait of goat’s prolificacy needs further studies.%采集5只多胎金堂黑山羊和5只单胎藏山羊在发情期的卵巢、垂体等组织样,进行Apaf-1和Apaf-2基因的cDNA克隆、序列分析,以及定量PCR技术对其mRNA进行组织表达量研究。结果表明,克隆出Apaf-1基因长度为3750 bp,编码1249个氨基酸, Apaf-2基因编码区全长为318 bp,编码105个氨基酸。这两个基因在两种山羊中序列相同,没有突变,且在5种组织中的表达均无差异。表明凋亡基因Apaf-1和Apaf-2在动物的进化中比较保守,与山羊多羔性状的相关性需要进一步研究。
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