In this study microwave-assisted petroleum ether/ethanol two-phase solvent extraction was applied to extract the essential oil from the fermentation broth ofTaiwanofunguscamphoratus, and the minimal inhibitory concentration(MIC)of the essential oil against 7 species of dermatophyte were determined. The optimized extraction conditions are as following:ethanol concentration 56%(v/v), petroleum ether concentration 30%(v/v), solid-to-liquid ratio 1∶50, microwave power 380 W and microwave time 90 s. The highest yield of extracted essential oil was 0.69%, and the MIC against the tested fungi were 5-20 mL/L. This extraction technology is efficient and time saving, moreover, the produced essential oil shows significant inhibitory effect on the growth of the tested dermatophyte.%采用微波辅助石油醚/乙醇双液相萃取技术提取樟芝发酵液中的挥发油，并考察了其对7种皮肤癣菌的最低抑制浓度（MIC）。优化的提取条件为：乙醇体积浓度56%，石油醚体积浓度30%，固液比1∶50，微波功率380 W，微波时间90 s。此时，挥发油的提取率为0.69%，真菌MIC为5-20 mL/L。微波辅助双液相萃取挥发油耗时短，提取率高，且获得的挥发油抗皮肤癣菌效果显著。
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