首页> 中文期刊> 《骨研究:英文》 >Inhibition of RgslO Expression Prevents Immune Cell Infiltration in Bacteria-induced Inflammatory Lesions and Osteoclast-mediated Bone Destruction

Inhibition of RgslO Expression Prevents Immune Cell Infiltration in Bacteria-induced Inflammatory Lesions and Osteoclast-mediated Bone Destruction

         

摘要

Regulator of G-protein Signaling 10(Rgs10)plays an important function in osteoclast differentiation.However,the role of Rgs10 in immune cells and inflammatory responses,which activate osteoclasts in inflammatory lesions,such as bacteria-induced periodontal disease lesions,remains largely unknown.In this study,we used an adeno-associated virus(AAV-)mediated RNAi(AAV-shRNA-Rgs10)knockdown approach to study Rgs10’s function in immune cells and osteoclasts in bacteria-induced inflammatory lesions in a mouse model of periodontal disease.We found that AAV-shRNA-Rgs10 mediated Rgs10 knockdown impaired osteoclastogenesis and osteoclast-mediated bone resorption,in vitro and in vivo.Interestingly,local injection of AAV-shRNA-Rgs10 into the periodontal tissues in the bacteria-induced inflammatory lesion greatly decreased the number of dendritic cells,T-cells and osteoclasts,and protected the periodontal tissues from local inflammatory damage and bone destruction.Importantly,AAV-mediated Rgs10 knockdown also reduced local expression of osteoclast markers and pro-inflammatory cytokines.Our results demonstrate that AAVshRNA-Rgs10 knockdown in periodontal disease tissues can prevent bone resorption and inflammation simultaneously.Our data indicate that Rgs10 may regulate dendritic cell proliferation and maturation,as well as the subsequent stimulation of T-cell proliferation and maturation,and osteoclast differentiation and activation.Our study suggests that AAV-shRNA-Rgs10 can be useful as a therapeutic treatment of periodontal disease.

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