微流控技术以其高通量、低消耗和集成化等优点成为蛋白质结晶微型化研究的重要手段。本文综述了基于微流控技术的蛋白质结晶技术和方法，主要包括微泵微阀、液滴( Droplet)、滑动芯片( SlipChip)以及液滴实验室( DropLab)等技术。此外，还针对当前膜蛋白在结构生物学研究中的重要地位，综述了应用于膜蛋白结晶的微流控技术的研究进展。%With the advantages of high throughput, low consumption and large-scale integration, microfluidics has proven to be a promising technique for protein crystallization and screening. This article reviews the recent progress of various microfluidic techniques successfully applied in protein crystallization, including PDMS-based valve and pump, droplet, SlipChip and DropLab. In addition, due to the high importance and great challenges of membrane proteins in structural biology, we further introduce the microfluidic techniques for crystallization of membrane proteins.
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