A lable-free aptamer biosensor was fabricated with the electropolymerized poly (thionine) as a redox electrochemical probe and gold nanoparticles as the immobilization matrix for aptamer. Electrochemical impedance spectroscopy was used to monitor the self-assembly process of the aptamer biosensor. The electrochemical behavior of the aptamer biosensor was studied by the cyclic voltammetry ( CV) and differential pulse voltammetry ( DPV). The biosensor shows a good linear range of 1. 0 pg/mL-500 ng/mL for thrombin, R = 0.998, with a detection limit of 0. 38 pg/mL. The biosensor has simple preparation procedure, high sensitivity and strong anti-interference ability.%采用电聚合法制备了聚硫堇氧化还原电化学探针,以金纳米粒子为固定核酸适配体的载体构建了非标记型核酸适配体传感器.用电化学阻抗谱对传感器的组装过程进行了监测,用循环伏安法和差分脉冲伏安法考察了传感器的电化学行为.结果表明,该传感器对凝血酶的检测在1.0 pg/mL ～ 500 ng/mL范围内呈良好的线性关系,相关系数为0.998,检出限为0.38 pg/mL.该传感器制备简单、灵敏度高且抗干扰能力强.
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