制备了蒙脱土修饰碳纤维电极, 研究了其对神经递质多巴胺(DA)及5-羟色胺(5-HT)的富集作用, 以及对负电性的代谢产物3,4-二羟基苯乙酸(DOPAC)、 5-羟吲哚乙酸(5-HIAA)及脑内大量存在的抗坏血酸(AA)排斥性能. 该电极具有很高的灵敏度、分辨率和抗干扰性, 对DA的检测下限达1.6×10-8 mol/L, 优于传统的Nafion修饰电极, 对5-HT的检测下限为1×10-7 mol/L. 在动物活体分析中, 使用该电极成功地检测了大鼠双侧颈总动脉结扎-再灌损伤时, 脑纹状体中神经递质DA浓度的变化.%The construction of montmorillonite-modified carbon fiber electrode is reported. The modified electrode shows a good sensitivity and selectivity due to its accumulation to cations and inhabitation to anions. The detection limit of DA and 5-HT are 1.6×10-8 and 1×10-7 mol/L, respectively, which are more sensitive than Nafion modified electrode. In-vivo voltammetry, the electrode has directly detected the dramatic variation of DA concentration in a rat brain with decreasing blood supply, while it effectively inhabits the oxidation of electroactive compounds and some neurotransmitter metabolites, such as AA, DOPAC, 5-HIAA.
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