采用高效液相色谱-二极管阵列检测法(HPLC-DAD)测定2、3年生不同月份(5月、6月、7月、8月和9月)采集的栽培宽叶羌活中绿原酸、阿魏酸、紫花前胡苷、补骨脂素、佛手柑内酯、欧前胡素、羌活醇和异欧前胡素的含量,考察采收时间和生长年限对栽培宽叶羌活主要活性成分含量的影响.结果表明,2、3年生栽培宽叶羌活各活性成分在返青及生长初期(5～6月份)含量较高,在生长旺盛的花果期(6～7月份)呈明显下降趋势,8月份达到最低水平,9月份又略有回升.2年生栽培宽叶羌活所测活性成分总含量在不同月份均低于3年生栽培宽叶羌活.生长年限和采收时间对宽叶羌活活性成分含量的积累具有重要影响,本研究对于宽叶羌活的进一步研究和开发应用提供了科学依据.%To investigate the influence of harvesting time and growth year on the content of themain active compounds in roots and rhizomes of cultivated Notopterygium franchetii H. de Boiss,the content of chlorogenic acid,erulic acid,nodakenin,psoralen,ber-gapten,imperatorin,isoimperatorin and notopterol in roots and rhizomes of 2 and 3 year old N. franchetii collected in different months(May to September)were determined by high-performance liquid chromatography(HPLC)coupled with photodiode array de-tector(DAD)using a C 18 column(TSK gel ODS-80Ts QA,250 mm×4. 6 mm i. d. 5 μm,Tosoh Co. Ltd. ,Japan). The results showed that the content of the active compounds in both 2 and 3 year old cultivated N. franchetii reached a rather high level in May and June,when it was the early growth period of this medicinal plant. Then,the content remarkably declined from June to July,during which time it entered the vigorous growth period. It then reached the lowest point in August and slightly rebounded in September. The total content of the active compounds in 2 year old cul-tivated N. franchetii was lower than that of 3 year old cultivated N. franchetii in each corresponding month. Both the harvesting time and growth year had a great influence on the accumulation of the active compounds in N. franchetii. The results will provide scientif-ic basis for further study and exploitation of N. franchetii.
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