Bovine serum albumin modified Cu nanoclusters( BSA-Cu NCs) were synthesized,and it was found that Cu NCs could en-hance the chemiluminescence( CL) emission of the luminol–H2 O2 system in an alkaline medium. The CL spectra and radical scav-engers were conducted to investigate the possible CL enhancement mechanism. Interestingly,tryptophan was found to enhance the CL emission of Cu NCs–luminol–H2 O2 system greatly,based on which,a novel CL assay for the detection of tryptophan was de-veloped with a linear range of 2. 0 × 10-7–10-4mol·L-1 and a detection limit as low as 6×10-8 mol·L-1.%合成了牛血清白蛋白修饰的铜纳米簇( BSA-Cu NCs),研究发现在碱性环境中,BSA-Cu NCs对鲁米诺-过氧化氢体系的发光信号有很好的增强作用。对铜纳米簇催化鲁米诺-过氧化氢化学发光的机理进行了研究,并发现色氨酸对鲁米诺-过氧化氢-铜纳米簇化学发光体系信号具有增强作用,基于此,建立了化学发光测定色氨酸的含量的方法。该方法对色氨酸的检测限为6×10-8 mol·L-1,线性范围为2．0×10-7~10-4 mol·L-1。
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