Ag2 S-MWNTs nanoconposites was prepared and then a Mb-Ag2 S-MWNTs-CHIT/GCE was fabricated. Based on the fabri-cated Mb-Ag2 S-MWNTs-CHIT/GCE,the direct electrochemistry and electrocatalytic properties of the immobilized Mb were investi-gated. Scanning electron microscopic and Transmission electron microscopic were used to characterize the morphology of the Ag2 S-MWNTs. Cyclic voltammetry and amperometry were used for studying the electrochemical behaviors of Mb. Uniform and stable Ag2 S was growing at the MWNTs surface,and the fabricated electrode showed a well-defined and quasi-reversible redox peaks and good electrocatalytic ability for hydrogen peroxide( H2 O2 ) reduction. The resulting modified electrode could detect H2 O2 in a linear range of 1. 0 × 10-6 ~2. 5 × 10-4 mol·L-1 and the detection limit was 3 × 10-7 mol·L-1 at a signal-to-noise ratio of 3. Ag2 S-MWNTs in-creased the direct electron transfer rate dramatically and the modified electrode can provide a good platform for the investigation of the third generation biosensors.%制备了硫化银-多壁碳纳米管( Ag2 S-MWNTs)纳米复合材料,构置了Mb-Ag2 S-MWNTs-CHIT/GCE,并研究了肌红蛋白( Mb)在该修饰电极上的直接电化学和电催化行为。采用扫描电镜和透射电镜表征了Ag2 S-MWNTs的形貌,利用循环伏安法对Mb的电化学行为进行研究。 Ag2 S能够均一、稳定的在MWNTs表面生长,所构置的修饰电极在PBS中出现一对峰形良好的、准可逆的氧化还原峰,并对过氧化氢( H2 O2)表现出良好的电催化作用,测定H2O2的线性范围为1．0×10-6~2．5×10-4 mol·L-1,检出限为3×10-7 mol·L-1(S/N=3)。 Ag2 S-MWNTs纳米复合材料能显著提高氧化还原蛋白质(酶)的直接电子传递速率,所构置的修饰电极可为制备基于蛋白质(酶)的第三代电化学生物传感器提供一良好的研究平台。
展开▼
