The interactions of terpyridine derivatives(L1-L3)and their Pt(Ⅱ)complexes(1-3)with human telomeric i-motif DNA were studied by UV-visible absorption titration,fluorescence titration,UV melting,and CD spectroscopy. The results show that these compounds can bind to i-motif DNA,and the affinity potential of complexes to i-motif DNA is larger than ligands,with the binding constants(Kb)of 3. 20(3. 80(105and 1. 20(1. 43(105M-1,respectively. UV-melting and CD spectral results indicate that the bind-ing of complex destabilizes the structure of i-motif DNA,while the binding of ligands fails to change the conformation and the melt-ing temperature of DNA.%本文通过荧光滴定、紫外可见吸收滴定、紫外熔点实验以及CD光谱研究了三联吡啶衍生物L1-L3及其Pt(Ⅱ)配合物1-3和人端粒i-motif DNA的相互作用。结果表明：配体和配合物均能与i-motif DNA相互作用,结合常数约为105 M-1,而且配合物的亲和作用强于配体。配合物的结合导致i-motif DNA的熔点温度降低并对其构象产生扰动。
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