考查不同Co-60辐照剂量对川贝活性成分的影响,为川贝的灭菌贮存和质量评价提供参考.采用HPLC法分析成分的变化.在不同辐射剂量照射后,川贝的化学成分的组成基本不变(各组分的保留时间tR不受辐照影响),但钴-60辐照剂量会对川贝药材某些组分的含量有不同程度的影响,在川贝的贮存和质量评价中应引起注意.%To provide guidance of ChuanBei storage and quality evaluation through examining different Co irradiation dose. The column Shim-pack VP-ODS( 150mmx4. 6mm,5. 0μm) was used. The mobile phase consisted of A(2. 5% of phosphoric acid sodium hydrogen and 10%sodium dihydrogen phosphate solution)and B( methanol) with a gradient elution,and the flow rate was l.0mL/ min. The detection wavelength were 200 nm, and the column temperature was 30°C. The composition structure of same original ChuanBei in different radiation dose was same based on retention time( tR ) ,but some components were affected by cobalt 60 irradiation doses. There are obvious effect on some active component content of ChuanBei by Cobalt 60 irradiation. This method can be used as a means of ChuanBei storage and quality assessment.
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