It is reported that pyritinol hydrochloride (Pyr) which is a drug containing hydroxyl group(s) is determined by using sodium 1,2-naphthoquinone-4-sulfonate (NQS) in this paper.The method is based on that in solution of pH 13.00,Pyr can catalyze the reaction between NQS and hydroxyl ion to form 2-hydroxy-1,4-naphthoquinone .Absorbency is linear maximal absorption wavelength of 454nm.The regression equation is A=0.02715+0.02837C(×105 mol L-1),with a regression coefficient of 0.9986(ε R.S.D.is 1.6%.The method reported has been applied to the determination of Pyr in pyritinol hydrochloride tablets and the average recoveries rate is in range of 98.0%~101%.%建立了1,2-萘醌-4-磺酸钠分光光度法测定含羟基药物盐酸吡硫醇的方法.研究表明,在pH=13.00的KCl-NaOH缓冲溶液中,盐酸吡硫醇能够催化氢氧根离子与1,2-萘醌-4-磺酸钠反应形成橙红色的2-羟基-1,4-萘醌,其最大吸收波长为454nm.盐酸吡硫醇浓度在3.2μg/mL~80μg/mL范围内与吸光度呈现良好的线性关系.线性回归方程为A=0.02715+0.02837C(×105 mol/L),线性相关系数r=0.9986.表观摩尔吸光系数、相对标准偏差(R.S.D.)和检测限分别为2.84×103L/mol/cm、1.6%和2.0μg/mL.通过对片剂中的盐酸吡硫醇含量进行测定,回收率在98%~101%.
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