本文提出了一种基于胶束增敏荷移反应测定红霉素的光度分析法.研究了在阴离子表面活性剂十二烷基硫酸钠(SDS)胶束体系中红霉素作为电荷供体与电荷受体水杨基荧光酮(SAF)之间的荷移络合物的光谱性质.发现SDS对该体系有增敏作用(ε=3.95×104 L·mol-1·cm-1提高近3倍),红霉素浓度在0.73mg/L～51.4mg/L范围内符合比尔定律,r=0.9999.本方法用于片剂中红霉素含量的测定,回收率为99.7%,9次测定的相对标准偏差为0.24%.%A new spectrophotometric method for the determination of erythromycin has been developed based on the charge-transfer reaction with micelle-sensitized.The spectrum characteristics of charge-transfer complex formed between erythromycin as the donor and Salicyl Fluorone (SAF) as the acceptor in SDS micellar system was studied.Experimental results show that the absorption of the complex was raised 3 times by SDS micellar system than that of non-micellar system.The molar absorptivity of erythromycin complex (ε= 3.95×104 L·mol-1·cm-1 at 519nm).Beer's law is obeyed from 0.73 to 51.4mg/L,r= 0.9999.The recoveries are 99.7%.The relative standard rndeviation is 0.24% (n=9).The proposed method has been applied to the determination of erythromycin in tablets with satisfactory results.
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